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B i o l u m i n e s c e n c e

B i o l u m i n e s c e n c e. Bio-(life) luminescence-(glows)- Glowing forms of life. By: Amber Guzman and Kali Matthias http://www.lifesci.ucsb.edu/~biolum. Why would living things want to glow???. To find food. 2. Attract a mate. 3. Scare away/ confuse predators. 4. See in the dark.

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B i o l u m i n e s c e n c e

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  1. B i o l u m i n e s c e n c e Bio-(life) luminescence-(glows)- Glowing forms of life By: Amber Guzman and Kali Matthias http://www.lifesci.ucsb.edu/~biolum

  2. Why would living things want to glow??? • To find food 2. Attract a mate 3. Scare away/ confuse predators 4. See in the dark 5. To communicate

  3. The genus Aglantha is bioluminescent, but the blue and purple colors in this photo are formed by iridescence of thin tissues in its bell. The thickness of the tissue and angle of light striking it can cause bright colors to show up. The orange color near the mouth is pigmentation, probably to mask luminescence or attract prey.

  4. The Lanternfish have species-specific patterns to the distribution of their light organs, they must be used to recognize potential mates. They likely use their luminescence for counterillumination as well.

  5. The deep-sea scyphomedusa Atolla vanhoeffeni it can produce an incredible perpetuated luminescence display when disturbed.

  6. Euphausia pacifica is a small vertically migrating species of krill.

  7. The rainbow colors on ctenophores are not bioluminescence. They are merely diffraction acting on the ambient light. This shallow-dwelling species, Beroe forskalii, produces a bright luminescent display when disturbed.

  8. This small squid in the genus Abraliopsis has several different types of light organs.

  9. What is GENETIC TRASFORMATION?? • Genetic Transformation means change caused by genes, and involves putting a gene into a living thing to change it’s characteristic.

  10. How is it used in the real world? • In medicine, doctors can hook the pGLO gene (glowing gene) onto a gene that is carrying AIDS and they will be able to follow it and see where it goes in the body.

  11. Key Terms

  12. Bioluminescent: Bio-(life) luminescent-(glows)- Glowing forms of life pGLO: Plasmid containing the GFP sequence and ampicillin resistance gene. Plasmid: A circular DNA molecule, capable of cloning itself carrying one or more genes. GFP (Green Fluorescent Protein): When added to a gene, gives this gene the ability to glow and be seen or identified under ultraviolet light and gives off energy in the form of visible green light.

  13. STEP 1 & 2 2 0 0 20 ul thawed competent cells into tube with DNA 3 ul pGLO DNA into tube 0 3 0

  14. STEP 3 & 4 Gently mix DNA cells by flicking the tube several times; tap the tube to get liquid to bottom Place tube on ice for 10 minutes Flick = Flick Flick

  15. STEP 4 Heat shock the cells at 420 C for EXACTLY 45 SECONDS; return to the ice.

  16. STEP 5 Add 80 ul of LB Broth to the cells and incubate the tube at 370 C for 15 minutes 80 ul LB Broth 0 8 0 FEED YOUR CELLS!!

  17. STEP 6 Plate 100 ul entire content of tube Agar plate

  18. ori araC bla GFP pGLO

  19. The End!

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