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Explore the differences between using Pathway Tools in Desktop vs. Web mode for bioinformatics research. Learn about advanced query tools, pathway prediction, editing options, and ongoing efforts to transition Desktop features to the Web. Get detailed guides on configuring the Web server and interacting with databases effectively.
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WWW Server Operation Markus Krummenacker Fred Gilham Bioinformatics Research Group SRI International kr@ai.sri.com gilham@ai.sri.com
WWW vs. Desktop http://biocyc.org/desktop-vs-web-mode.shtml WWW: Comparative Genomics Tools (HTML Tables) Advanced Query Tool, using BioVelo BLAST Searching Desktop: Pathologic Prediction of new PGDBs Pathway Hole Filler All Editing Tools for Curation Many Overview Highlighting Options Ongoing effort to move Desktop features to Web
Starting WWW Server: Basics Configured by ptools-init.dat parameters Required: (the values are used for generating URLs) WWW-Server-Hostname WWW-Server-Port Description: UG Section 2.1.1 Organism Selection Start WWW Server: pathway-tools -www Windows—just click on “Web Server” desktop shortcut
WWW Server: Advanced Several more ptools-init.dat parameters Some exotic parameters supplied as command-line args Command-line args override ptools-init.dat Description: UG Section 2.3.1 Database Links: Link to/from PGDB and external web site UG section 8.5.10 Manual and bulk
Unsupervised Web Server Operation Pathway Tools needs some X Server Xvfb is a display-less X Server Use screen for starting pathway-tools Description: http://bioinformatics.ai.sri.com/ptools/web-logout.html
Lab Exercise Start up Pathway Tools in web server mode Connect to it with your browser: http://localhost:1555 Oops! Too many organisms! Fix so it shows ONLY the HPY organism Do it on the command line -www-publish hpy Edit the ptools-local/ptools-init.dat file WWW-Publish entry
Setup MySQL PGDBs • Configure mysql server • http://bioinformatics.ai.sri.com/ptools/installation-guide/released/mysql.html • User account that will update curation DB • Set in ptools-init.dat
Configure Ptools • Configure ptools-init.dat • Convert FileKB to MySQL via Pathologic
PGDB Snapshot • Pathologic Backup DB to File • Upgrade DB version