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Bacteriology Final Section

Bacteriology Final Section. Created by: Laura Wilson. “Soft skin”, no cell wall, smallest prokaryotes known:. Mycoplasma. What are the properties of Mycoplasma?. Pleiomorphism Plasticity Resistance to antibacterials (penicillins) Sensitivity to osmotic shock

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Bacteriology Final Section

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  1. Bacteriology Final Section Created by: Laura Wilson

  2. “Soft skin”, no cell wall, smallest prokaryotes known: • Mycoplasma

  3. What are the properties of Mycoplasma? • Pleiomorphism • Plasticity • Resistance to antibacterials (penicillins) • Sensitivity to osmotic shock • Requirement for complex media, esp. sterols and fatty acids • Close associations w/ cell surfaces

  4. What are the common sites for Mycoplasma? • Mucosal surfaces of respiratory, digestive, and genital tracts, and mammary gland

  5. T/F • Many Mycoplasmas can survive for long periods of time outside host

  6. True

  7. What would you stain with for Mycoplasma? • Romanowsky stains used (Giemsa, Wright’s, etc)

  8. Mycoplasmas are: • A. Strict anaerobes • B. Aerobic • C. Facultative anaerobes—prefer CO2 • D. Microphilic

  9. C. Facultative anaerobes—prefer CO2

  10. Where are antigens for Mycoplasma located? • In cell membrane and intracellularly

  11. What are the bacterial virulence factors for Mycoplasma? • Intimate contact w/ host cells • Destroy inactive cilia • Various extracellular proteins: • --cytotoxic glycoproteins • --hemolysins • --Proteolytic enzymes

  12. What are the host factors for Mycoplasma? • Stress • Debility • Intercurrent infections

  13. What is the cause of “Enzootic pneumonia pigs/swine”: • Mycoplasma hyopuenmoniae

  14. What are the clinical signs of “Enzootic pneumonia pigs”? • Unproductive cough, unthrift, weight loss

  15. What is the cause of polyserositis and arthritis in pigs– pigs 3-10 wks are nasal carriers? • Mycoplasma hyorhinis

  16. What is the cause of arthritis in pigs following stress– pigs 10-24 weeks old are nasal carriers: • Mycoplasma hyosynoviae

  17. What is the cause of Contagious Bovine Pleuropneumonia (CBPP) in cattle? • Mycoplasma mycoides

  18. T/F • Mycoplasma mycoides is found throughout the US causing Contagious Bovine Pleuropneumonia

  19. False • Not in US, but in Central Africa, M.E. and China

  20. What does Mycoplasma bovis cause? • Mastitis • Pneumonia in calves • Arthritis • Genital infections

  21. What is the cause of septicemia in goats only, found in the tropics, with a possible high mortality rate? • Mycoplasma mycoides mycoides

  22. Where does the septicemia occur in goats with Mycoplasma mycoides mycoides? • Lungs • Udder • Joints • Eyes • Tonsils

  23. What is the cause of Contagious Caprine Pleuropnemonia (CCPP)—not occuring in US: • Mycoplasma mycoides carpi

  24. What is the cause of “pinkeye” – Infectionous Keratoconjunctivitis in sheep and goats? • Mycoplasma conjunctivae

  25. What are the symptoms of Mycoplasma conjunctivae in goats/sheep similar to in cattle? • Moraxella bovis

  26. What is the cause of contagious agalactiae in sheep/goats, in US, causing severe mastitis and agalactia shortly after partuition, often leads to arthritis: • Mycoplasma agalactiae

  27. What is the cause of airsacculitis and skeletal abnormalities in young turkeys? • Mycoplasma meleagridis

  28. What is the cause of Chronic Respiratory Disease (CRD) in chickens and Infectious sinusitis in turkeys: • Mycoplasma gallisepticum

  29. What clinical signs does Mycoplasma gallisepticum cause in chickens and turkeys: • Inflamed sinuses, trachea, lungs, and airsacs • Turkeys show swellings of intraorbital sinuses

  30. What is the cause of Infectious Synovitis in Chickens and Turkeys? • Mycoplasma synoviae

  31. What treatments should be used with Mycoplasma infections? • Erythromycin • Tylosin • Tiamulin • Fluoroquinolones • Aminoglycosides • Tetracyclines

  32. Small, coccoid, obligate intracellular parasites, Gram – (not stained well): • Orders: • Chlamydiales • Rickettsiales

  33. Prokaryotes, have cell wall but lacking muramic acid, no arthropod vector required: • Chlamydiales

  34. What would you stain a Chlamydiale with? • Giemsa, modified acid fast– Modified Ziehl-Neelson

  35. What is the main pathogenesis for Chlamydiales? • Resist phagocystosis

  36. What is the cause of “Enzootic Abortion in Ewes” EAE: • Chlamydophila abortus

  37. T/F • Pregnant woman are at high risk from Chlamydophila abortus

  38. True

  39. What is the cause of “psittacosis” in birds and man? • Chlamydophila psittaci

  40. T/F • Chlamydophila psittaci is a reportable disease

  41. True

  42. What are the clinical signs of Chlamydophila psittaci in turkeys? Ducklings? • Turkeys: • Lethargy, decreased appetite, resp. distress • Ducklings: • Nervous signs w/ trembling, incoordination, and convulsions

  43. What is the cause of Feline pneumonitis? • Chlamydophila felis

  44. What are the clinical signs from Chlamydophila felis? • Conjunctivitis w/ upper resp. tract inf.

  45. Chlamydophilus felis is highly contagious, and effects mainly: • Neonatal kittens

  46. Small, obligate intracellular bacteria, parasites of arthropods, stain w/ Romanowsky stains: • Rickettsiales

  47. What is the cause of “Rocky Mountain Spotted Fever”, effecting humans and dogs mainly: • Rickettsia rickettsii

  48. What is the cause of “Q fever” mainly in humans: • Coxiella burnetti

  49. What are the symptoms of Coxiella burnetti in man? • Fever • Headaches • Sweating • Pneumonia • Endocarditis • Hepatitis

  50. T/F • Arthropod vectors are needed for Coxiella burnetti

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