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Bacteriology 7th Practical

PRESS F1 FOR GUIDEANCE. Bacteriology 7th Practical. MFSH 2003. Contents. Enterobacteria, Vibrio, Campylobacter & Pseudomonas Enterobacteria 3 Station 1: Gram stain of Enterobacteria 4 Station 2: NLF + LF on Mc Conkey 5 Station 3: Klebsiella on Mc Conkey 6

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Bacteriology 7th Practical

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  1. PRESS F1 FOR GUIDEANCE Bacteriology 7th Practical MFSH 2003

  2. Contents Enterobacteria, Vibrio, Campylobacter & Pseudomonas Enterobacteria 3 Station 1:Gram stain of Enterobacteria 4 Station 2: NLF + LF on Mc Conkey 5 Station 3: Klebsiella on Mc Conkey 6 Station 4: E.coli on Mc Conkey 7 Station 5: Pure LF & Pure NLF on both Mc Conkey & DCA 8 Station 6: Proteus on Blood agar 9 Station 7: Selenite F broth 10 Station 8: Indole production 11 Station 9: H2S Production 12 Station 10: Widal test 13 Station 11: Treatment & diseases 14 Vibrio 15 Station 11: Gram stain of Vibrio cholera 16 Station 12: Growth on TCBS 17 Station 13: Growth on TCBS & alkaline peptone water 18 Campylobacter 19 Station 14: Gram stain of Campylobacter 20 Station 15: Urease Production 21 Pseudomonas 22 Station 16: Growth on Nutrient agar 23 Station 17: Sensitivity test 24 Station 18: Growth on blood media 25 Station 20: Treatment and diseases 26

  3. Enterobacteria

  4. Station 1 E.g. E.coli Klebsiella Proteus Shigella Salmonella and others. Non lactose fermenters

  5. Station 2 NLF LF NLF= non lactose fermenters LF= lactose fermenters

  6. Station 3 Large mucoid colonies (pinkish / reddish)

  7. Station 4 Pink colonies

  8. Station 5 Pinkish Pale LF (e.g. E-coli & Klepsilla) N.B. it is E-coli here “Shape of colonies” NLF (e.g. Salmonella, Shigella) Pale Pinkish NLF LF

  9. Station 6

  10. Station 7 Samonella and Shigella

  11. Station 8

  12. Station 9 Black e.g. shigella

  13. Station 10 1/10 1/20 1/40 ……… 1/640 1/10 1/20 1/40 - test - + + test 1/10 1/20 1/40 1/80 1/160 1/320 - + There is more than 4 folds increase in the titer of antibodies against Salmonella typhi “320/40= 8”, this means acute infection.

  14. Station 11 • Salmonella • Infection:- • 1- typhoid fever • 2- food poising “meat, poultry & egg”  diarrhea • This usually happens when people eat in public places “Pakistan, Sudan & Egypt” • Treatment:- • In enteric fever  “chloramphenicol”, if resistant  “ciprofloxacilin” Escherichis coli Infection:- 1- urinary tract infection 2- diarrhea Treatment:- requires antibiotic testing, but most common are 1-”Ampicilin” 2-”Aminglycosides” • Shigilla • Infection:- • dysenteriae (enterotoxin, cytotoxic & neurotoxic)  bloody, mucoid & small stools with fever • Treatment:- • Fluid replacement & “Ampicillin” Klebsiella Infection:- urinary tract infection Treatment:- beta lactamase producing “Aminglycosides” is used • Proteus • Infection:- • urinary tract infection, by the urease the urea is split to produce ammonia  “alkaline urine”

  15. Vibrio

  16. Station 12 E.g. Vibrio Cholerae

  17. Station 13

  18. Station 14 Clear Mentioned previously Turbid aureus

  19. Campylobacter

  20. Station 15 (vibrio) E.g. Campylobacter jejuni

  21. Station 16 Helicobacter pylori

  22. Pseudomonas

  23. Station 17 No pigment

  24. Station 18 Pseudomonas is resistant to many antibiotics “but sensitive to amino glycosides (e.g. gentamicin) and some others” Sensitive Sensitive

  25. Station 19 Green orange Note that Pseudomonas is strictly aerobic

  26. Station 20 • Vibrio cholera • Infection:- • cholera (exotoxin  persistent and excessive secretion of isotonic fluid by intestinal mucosa) • Treatment:- • “Erythromycin” • Campylobacter jejuni • Infection:- • Food poisoning “Chicken”  diarrhea. • Treatment:- • “Erythromycin” • Helicobacter pylori • Infection:- • Gastric and duodenal ulcerations • Treatment:- • Triple therapy: “Omeprazole” + “Amoxycilin” + “Metronidazole” • Quadruple therapy: Same + proton pump inhibitors • Pseudomonas • Infection:- • Gastric and duodenal ulcerations • Treatment:- • “Erythromycin”, “Amoxycilin”

  27. قصائد غزل مصرية حبك بطحني عالحشيش ... ومن غيرك مأقدرش أعيش ... أحنا جوز جزم وفردة وحدة م تمشيش .. قطعني حتة حتة وأرميني لأي قطة ... أنا كنت بأحب واحدة دلوقتي بأحب ستة دعواتكم يا شباب

  28. I hope it was useful. A word can change the outcome of many things. So, if you either liked or didn’t like this project, why keep your thoughts to yourself ? Send us your comments and ideas to COORDINATOR1422@YAHOO.COM, It will mean too much to us, “even if it was just one word” THANX M. F. Shaheen 2003

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