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Ch. 30 Capillary Electrophoresis, Capillary Electrochromatography and Field - Flow Fractionation

Ch. 30 Capillary Electrophoresis, Capillary Electrochromatography and Field - Flow Fractionation. Electrophoresis = ? Capillary Electrophoresis = ?. 電 泳.

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Ch. 30 Capillary Electrophoresis, Capillary Electrochromatography and Field - Flow Fractionation

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  1. Ch. 30 Capillary Electrophoresis, Capillary Electrochromatography andField - Flow Fractionation Electrophoresis = ? Capillary Electrophoresis = ?

  2. 電 泳 Electrophoresis: a separation method based on the differential rate of migration of charged species in a buffer solution across which has been applied a dc electric field. Q / d : charge-to-size ratio 1948 Nobel Prize Swedish chemist Serum proteins

  3. Slab electrophoresis Bio macromolecules: Proteins (enzyme, hormones, antibodies) Nucleic acids (DNA, RNA) 平板 L. Stryer, Biochemistry, 4th ed., W. H. Freeman, 1999, p. 47.

  4. F. Rouessac and A. Rouessac, Chemical Analysis, Wiley, 2000, p.112

  5. 毛細管電泳 Human Genome: sequence for human DNA

  6. Systematic apparatus for capillary electrophoresis 25 ~ 75 μm i.d. ~ 50 cm long 15 ~ 30 kV G. D. Christian, Analytical Chemistry, 6th ed., John Wiley, 2004, p. 632 vs. slab eletrophoresis: no joul heating effect

  7. FIGURE 30-1Schematic of a capillary electrophoresis system.

  8. D. C. Harris, Quantitative Chemical Analysis, 6th ed., 2003, p. 655.

  9. Acts as a pump 帶動整體溶液 buffer solution e.g. Na+Ac- Microfluid 微流體 電 滲 流 FIGURE 30-2Charge distribution at a silica-capillary interface and resulting electroosmotic flow. electrical double layer OH pH > 3 O- Si Si

  10. + = - + > - D. C. Harris, Quantitative Chemical Analysis, 6th ed., 2003, p. 658.

  11. 電滲流 - + In general, νeo > νe + 電泳 FIGURE 30-4Velocities in the presence of electroosmotic flow. The length of the arrow next to an ion indicates the magnitude of its velocity; the direction of the arrow indicates the direction of motion. The negative electrode is to the right and the positive electrode to the left of this section of solution. Separation is based on differences in electrophoretic mobility. charge / size migration velocity 淨移動速率

  12. parabolic flat FIGURE 30-3Flow profiles for liquids under (a) electroosmotic flow and (b) pressure-induced flow. electroosmotic hydrodynamic + - pump

  13. pump D. C. Harris, Quantitative Chemical Analysis, 6th ed., 2003, p. 658.

  14. ms ms D. C. Harris, Quantitative Chemical Analysis, 7th ed., 2007.

  15. D. C. Harris, Quantitative Chemical Analysis, 6th ed., 2003, p. 656.

  16. van Deemter equation: D. C. Harris, Quantitative Chemical Analysis, 6th ed., 2003, p. 656.

  17. Advantages of CZE: • High separation efficiency (N > 105) • Low reagent consumption • Low sample consumption (< 10 nL)

  18. Sample introduction in CZE: • Electrokinetic injection • Pressure injection • a. pressure • b. vacuum • c. height difference (gravity)

  19. TABLE 30-1Detectors for CE (UV/Vis)

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