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Bisulfite Sequencing and MSP Analysis of CASP8 Promoter in Cancer Cell Lines Treated with 5-Aza-dC

This study investigates the effects of 5-aza-2'-deoxycytidine (5-aza-dC) on the promoter methylation and expression of the CASP8 gene in various breast cancer cell lines, including MCF-7, MB231, and SKBR3. Using methylation-specific PCR (MSP) and bisulfite sequencing, we assessed the methylation status and compared the relative levels of maspin as a potential tumor suppressor. The findings reveal differential responses to 5-aza-dC treatment, contributing to our understanding of epigenetic regulation in breast cancer.

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Bisulfite Sequencing and MSP Analysis of CASP8 Promoter in Cancer Cell Lines Treated with 5-Aza-dC

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  1. 5-aza-dc - + - + - + - + - + - CASP8 b-actin 14 12 10 5-aza-dc - - + + - - M UM M UM M UM 8 CASP8 Bisulfite sequence CASP8 promoter MSP MCF-7 Water 6 CASP8 MSP Bisulfite sequence +51 4 -744 MB231 Water 2 CASP8 MSP MCF-7 5-aza-dc - - + + - - SKBR3 Water MCF-7 +5-aza-dc M UM M UM M UM 0 CASP8 maspin MSP MSP MB231 Water MCF7 CASP8 MB231 +5-aza-dc MSP maspin MSP Relative level of maspin (adjusted with 18S) Water MB231 SKBR3 MCF10 Water maspin MSP SKBR3 +5-aza-dc SKBR3 Water MCF-7 MB231 SKBR3 C A MCF-7 MB231 SKBR3HCC1837MCF10 Water +200 -800 B E MCF-7 MB231 SKBR3 HCC1937 - + D 5-aza-dc - + - + - + HCC1937 Water Caspase-8 β-actin F

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