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AP LAB 4

AP LAB 4. Photosynthesis Lab. What does the spectrophotometer measure? What did the chloroplasts do in the lab? What purpose does DPIP serve in the lab? How did DPIP behave in the lab? What molecule is DPIP replacing? What is the source of electrons to reduce DPIP?

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AP LAB 4

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  1. AP LAB 4

  2. Photosynthesis Lab • What does the spectrophotometer measure? • What did the chloroplasts do in the lab? • What purpose does DPIP serve in the lab? • How did DPIP behave in the lab? • What molecule is DPIP replacing? • What is the source of electrons to reduce DPIP? • Explain the results in each setup? • Unboiled/Dark • Unboiled/Light • Boiled/Light • No chloroplasts

  3. Chromatography Rf values are known and can be used to identify pigments (they are specific)

  4. Pigments Accessory Primary Chlorophylla Chlorophyll b Carotenoids Carotene Xanthophyll

  5. Rf Values • It is a ratio • .20 = x / 54nm • X = ~ 11mm

  6. Absorption Spectrum

  7. Spectrophotometer Measures light transmittance/absorbance

  8. DPIP – e- acceptor Changes from blue to colorless as it is reduced

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