Effective Date: 04/01/2013

1. KENTUCKY WATERSHED WATCH RECERTIFICATION – PHASE 1 Effective Date: 04/01/2013

2. Training and Certification Workshops • Phase 1 • Field Chemistry Assessment • Grab Sample Collection • Phase 2 • Biological Assessment • Habitat Assessment

3. Phase 1Watershed WatchRecertification Goals for this module • Refresh your skills • Highlight changes to sampling procedures • Distribute NEW flip books • Check out any replacement equipment you need • Complete essential recertification paperwork

4. Active Watershed Watch Sampling Sites

5. How we’re set up: Watershed Watch In Kentucky Board of Directors Basin Steering Committee or Board of Directors Sampling Event Coordinator Lab Manager Science Advisor(s) Area coordinator Area coordinator Area coordinator Watershed Watch Trained Sampler Watershed Watch Trained Sampler Watershed Watch Trained Sampler Watershed Watch Trained Sampler

6. Goal:Get people into the real world to see first hand the condition of their streams.

7. Watershed Watch provides you the tools and information you need to educate and inform You Family Friends Neighbors Community Local Leaders Resource Management Agencies

8. How you can use your information • Personal decision making • Local action • Grant applications • Watershed planning • Assessment screening • Enforcement efforts • Scientific research Data usage depends on the quality control applied…

9. Standard Operating Procedures (SOP) For good Quality Assurance of results, we require: • Training workshops for certification • Standard methods of collection • Standard methods of analysis • Full chain of custody and/or forms • Careful recordkeeping!

10. Standard Operating Procedures (SOP) To ensure standard methods of collection, always use flip cards when sampling:

11. SOP Statement This presentation is based on the “Kentucky Watershed Watch Field Chemistry Sample Collection Standard Operating Procedure WWSOP01000” and is available from wwky.org

12. Field Chemistry Standard Operating Procedures (SOP) NEW Flip Book

13. Pre-sampling Information • You will receive for sampling: • Instructions • Transport information • Chain of Custody Records • Gloves (disposable) • Sample containers • Preservative (if required) • You will receive materials from program coordinators or by mail • Read instructions upon receipt! (Critical info on sample times and delivery included) • Inspect all sample and preservative containers upon receipt for damage or breakage

14. Be Safe! • •During high flow, sampling should be postponed until baseline conditions exist – do not sample in any unsafe conditions • •Waders and specialized wading boots can be utilized • •Use extreme caution when wading in streams above the knee • •Samplers should exhibit caution in and around streams to reduce the threat of a falling injury • •Secure permission from landowner • •Be aware of increased risk for frostbite or hypothermia in cold temperatures • •Do not stick hands or feet under large objects. Exercise caution when handling aquatic life. Some organisms pinch, bite, etc. • All water bodies have the potential to be contaminated • Notify others of your whereabouts and carry a cell phone • Use caution and be aware of your surroundings and location

15. Equipment • You will need to purchase a gallon of distilled water for use during sampling events • All field chemistry sample containers must be rinsed with distilled water after each test

16. Equipment • Use your Waste Container to • dispose of all used chemicals • After rinsing field chemistry • sample containers, dispose of • rinse water in Waste Container • After sampling event, flush • contents of Waste Container • into a system that goes to a • wastewater treatment plant

17. Equipment • Store in a cool, dry place (climate controlled) • NOTa hot car or freezing garage! • Store out of reach of children and pets • In addition: • Chemicals should be replaced every three years at • your recertification training • Kits have enough chemical for 50 tests! • (use them all you want!) • Should you need replacement chemicals or • glassware before then, let us know

18. Equipment Chemical Safety • There are hazardous chemicals in these kits! • Dissolved Oxygen (DO) test uses a strong acid and alkali (like bleach) • pH Test chemical is flammable • Read Material Safety Data Sheets (MSDS) for • further information on chemical safety • Dispose of used chemicals in plastic waste • container at stream and then flush into municipal • wastewater treatment system (no septic systems) • Trade in old chemicals for proper disposal when • you receive replacement chemicals

19. Equipment Chemical Safety • Proper attire for performing tests • Gloves (disposable) • Eye protection

20. Equipment Chemical Safety • What to do if you get a chemical on you • For emergency first aid in the field, flush • affected area with large amounts of water • and seek medical attention if necessary • Follow the first aid procedures found on • the Material Safety Data Sheets (MSDS)

21. Sampling Procedure Field Observations and Water Chemistry Assessment • Enter stream at a downstream location • Walk upstream to avoid disturbing bottom • Perform your observations and tests just below your • grab sample location

22. Field Observations Flow Rate 0-5 Point Scale • 0 = Dry • 1 = Ponded • 2 = Low • 3 = Normal • 4 = Bank Full • 5 = FLOOD! • Do not sample • during these • flow conditions

23. Field Observations 48 Hour Rainfall Use Weather Channel, local paper or rain gauge Use any of these websites OR www.kymesonet.org/ www.cocorahs.org/state.aspx?state=ky wwwagwx.ca.uky.edu/cgi-bin/ky_clim_data_www.pl water.weather.gov/ To determine 48 hour rainfall in inches

24. Additional Field Observations • Site coordinates and Photos • GPS (decimal degrees – 5 decimal places) • OR Digital map (KGS WW Site - http://kgs.uky.edu/kgsmap/krww/viewer.asp • or Google Earth) • Take photos upstream and downstream of sampling point – with recognizable landmarks; should be taken annually during Summer sampling event • Label photos: “Site#upstream” and “Site#downstream” • Submit information to Water Watch office Email: OllieTheOtter@ky.gov • Phone: 1-800-928-0045

25. Field Measurements • For Field Chemistry tests: • pH • Dissolved Oxygen • Conductivity • (does not include Temperature) • Put on gloves • Collect water just below your grab sample location • Rinse bottle, tube or cup and any associated caps or • lids with stream water three times (fill container ¼ full of • stream water, cap, shake, and discard downstream) • Point mouth of bottle, tube or cup directly at the surface • of the water, submerge entire container to mid-depth of • stream, scoop upstream and fill to appropriate level

26. Field Measurements Conductivity Equipment • Meter Calibration • Must be calibrated for • each sampling event • Calibrate to 720 µS • (standard solution is • 718 µS) New Meter Old Meter

27. Field Measurements Conductivity Equipment Watershed Watch Chain of Custody Record • Meter Calibration • Record meter calibration • date on COC • Must be calibrated • within 24 hours prior • to sampling event

28. Field Measurements Conductivity Equipment – New Meter • New Meter Calibration • Display • Hold/enter • Calibrate • Power • Calibration Standard

29. Field Measurements Conductivity Equipment

30. Field Measurements Conductivity Equipment – Old Meter • Old Meter Calibration • Display • Hold • On/off • Calibration Standard

31. Field Measurements Conductivity Equipment Increase Decrease

32. Field Measurements Conductivity Equipment • Calibration (Old Meter): • Twist open lid on wider end (battery compartment) • Place narrower end (electrodes) in calibration standard • Press ON/OFF button and wait for display to stabilize • When viewed from top, hold meter with white buttons on left • Press the Increase (top) or Decrease (bottom) button until display reads 720 µS • When display flashes 3 times, then shows ENT, the meter is calibrated • Replace lid

33. Field Measurements Water Conductivity Test Procedure • Fill sampling container halfway with stream water • Press Power (ON/OFF) button and allow to stabilize • to 0 in air • Place meter in sample, • stir once, and wait for • reading to stabilize • Record result in µS • Rinse container and • narrower end of • meter (electrodes) • with distilled water

34. Field Measurements Remember to rinse the narrower end of meter (electrodes) and sampling container with distilled water!

35. Field Measurements Water Temperature Test Procedures • Place thermometer at mid-depth of stream in location with constantly flowing water • Leave thermometer • there until reading • stabilizes • Record temperature in • degrees Celsius (°C)

36. Grab Sample SOP This presentation is based on the “Kentucky Watershed Watch Stream Grab Sample Collection Standard Operating Procedure WWSOP02000” and is available from wwky.org

37. Grab Sample Standard Operating Procedures (SOP) NEW Flip Book

38. Pre-sampling Information Watershed Watch Chain of Custody Record The sampling containers are pre-marked with a unique Site Number The Site Number should match the number on the “Chain of Custody Record”

39. Pre-sampling information Ireceived a bottle with a site number I don’t recognize! Check the documents in your packet, you may have been randomly assigned to collect a blankor a duplicate

40. Pre-sampling Information • Blank - A sample bottle filled while at the stream site with distilled water • Serves as a check for possible contamination during sample handling or processing at the lab • DO NOT indicate that the sample is a blank – the lab should not know

41. Pre-sampling Information • Duplicate - A sample bottle filled with stream water at your site at the same time as your regular sample • Serves as a check for variability among samples that should yield approximately the same lab results • DO NOT indicate that the sample is a duplicate – the lab should not know

42. Collection Procedures Sample collection location Sample in an area of the stream where the flow is concentrated and where you can completely submerge the bottle without touching the bottom NOTE: It is important that you collect your sample at the same location each time

43. Collection Procedures Concentrated Flow Location

44. Collection Procedures Grab Sample Collection • Enter stream at a downstream location • Walk upstream to avoid disturbing bottom • Collect sample at the same location each time in • the middle of your concentrated flow location • and at mid-depth of stream

45. Collection Procedures ForE. coli grab sample collection: • Put on disposable gloves • DO NOT RINSE BOTTLE • Point mouth of bottle directly over surface of water, dip • and scoop bottle upstream beneath water surface, and • through water column in one swift motion to fill above • line marked on bottle • If preservative is in bottle, take caution to angle mouth so • as not to spill any while collecting sample • Cap container, leaving at least a small airspace at top to • allow lab mixing • Place in wet ice immediately

46. Collection Procedures For grab sample collectionother thanE. coli: • Put on disposable gloves • Rinse bottle with stream water three times • (fill bottle ¼ full of stream water, cap, shake, and discard • downstream) • Point mouth of bottle upstream (against the flow), • submerge entire bottle to mid-depth of stream, • scoop and fill • Rinse cap in stream and cap bottle • Add preservative (if necessary) and place in wet ice

47. Preservation • Samples must be kept immersed in wet ice • (but not frozen) until received by laboratory • Some samples may require other preservatives • (check your sample event packet)

48. Preservation Sample Holding Time: • length of time a preserved sample can be stored • after collection and before it is analyzed without • significantly affecting the results • from the minute you collect a sample, the • clock starts ticking on the holding time • varies for different types of analysis • established by the US Environmental • Protection Agency

49. Preservation Sample Holding Times E. coli= 6 hours maximum Other thanE. coli= Follow holding time instructions received in your sampling event packet

50. Recordkeeping Volunteer entries on the Chain of Custody Watershed Watch Chain of Custody Record • Date and Time of sample collection • Field Observations and Measurements • Date and time of sample • Relinquished/Received • must be identical Cross through any mistakes and initial – do not erase