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Micronucleation as genotoxic biomarker

Micronucleation as genotoxic biomarker. Micronucleation incidence in professionally exposed subjects to ionizing radiations in correlation with smoking. Mihalache D., Preoteasa V., Petrescu A, Codorean E, Obreja D. Victor Babes National Institute of Pathology, Bucharest, ROMANIA.

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Micronucleation as genotoxic biomarker

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  1. Micronucleation as genotoxic biomarker Micronucleation incidence in professionally exposed subjects to ionizing radiations in correlation with smoking Mihalache D., Preoteasa V., Petrescu A, Codorean E, Obreja D. . Victor Babes National Institute of Pathology, Bucharest, ROMANIA Aim: assessment of impact environmental factors,(genetic and life style related) upon genetic material stability in human populations. Material and methods: On total human venous blood cultivated on RPMI culture medium supplemented appropriately CBMN technique (M. Fenech.,2000). was applied. The culture was sacrificed at 72 hours and was fixed adequately after the hypotonic shock. Cells spread on glass slides were Giemsa stained and examined on a Nikon E 600 optical microscope with subsequent image acquisition. 1000 binucleated lymphoid cells ( scoring micronuclei) per case having intact membrane and cytoplasm were scored and statistically analyzed by Student’s “t” test. • Results: Micronuclei were examined on samples from 60 subjects divided in 2 lots of 30 de subjects: • “smokers” 10 controls and 20 radiation exposed subjects • “nonsmokers” comprising also 10 controls and 20 radiation exposed subjects. Fig 1, 2, 3. Micronuclei in binucleated lymphoid cells. CBMN method CONCLUSIONS Micronucleation analysis by gender shows the following: 1. in control males, smokers show an increase in micronuclei numbers 2. in professionally exposed subjects, more pregnant in females, increased genomic sensitivity is expressed by an increased frequency micronuclei 3.in females, micronuclei incidence correlates with smoking and age.

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