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Making Sense of our Restriction Digests

Explore the intricacies of PvuII digest results for plasmids, uncovering uncut lane bands, determining insert sizes, identifying PvuII sites, and comparing results with PCR findings. Learn how to deduce insert sizes from a 20JM4.10 digest and interpret gel run data.

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Making Sense of our Restriction Digests

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  1. Making Sense of our Restriction Digests Or not…

  2. Theoretical PvuII digests of plasmids p. 2B-10

  3. Why are there 3 bands in the uncut lane? p. 2B-10

  4. What is the size of the insert? 2.9 Kb 700 bp - 700 bp = 0 bp p. 2B-13

  5. Is there a PvuII site in the insert Are there multiple bands? p. 2B-13

  6. Do the results from the digest and the PCR agree? 700 bp - 700 bp = 0 bp 200 bp - 200 bp = 0 bp p. 2B-14

  7. How do we figure out the insert size from the 20JM4.10 digest? 2900 bp 1200 bp 400 bp 1200 bp + 400 bp-700 bp = 900 bp

  8. Section 12 ResDig Gel Run 9/28

  9. Section 34 ResDig Gel

  10. Section 58 Res Digest gel

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