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Small RNA and Cyanobacteria

Small RNA and Cyanobacteria

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Small RNA and Cyanobacteria

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Presentation Transcript

  1. Small RNA and Cyanobacteria

  2. Characteristics of Cyanobacteria • Aquatic • Photosynthetic • Usually unicellular

  3. Atmosphere Chloroplast Importance of Cyanobacteria

  4. What is sRNA? • Encoded in the intergenic regions

  5. Why do we want to learn about sRNAs? • To predict them in any genome of an organism • To experimentally analyze their function • To control gene expression

  6. Why have few sRNAs been found? • Very small (usually 40-300 nt) • Less sensitive to point mutations • Function at the 2º structure level • Do not encode protein sequence • No coding signals (start/stop codon)

  7. How Biolingua can search for sRNAs in cyanobacteria? • Compare the sequences of intergenic regions by BLAST • These results will include both an e-value and a percent identical score • Run the similar sequences from multiple organisms thru RNAz, which predicts if an RNA exists by its 2º structure

  8. To further verify an sRNA… • M-Fold: takes a potential RNA sequence and incorporates its structure • Experiments: test by northern blot analysis

  9. Acknowledgements: Jeff Elhai and Dr. You Chen