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Innovative Cell-Based Bio-Indicator to Enhance Meat Freshness Monitoring Beyond Sell-By Dates

This project addresses the limitations of traditional printed sell-by dates by introducing a cell-free bio-indicator that monitors the exposure of meat to environmental factors, especially temperature. Fresh meat is highly perishable, and studies show that improper temperature management during transportation and storage leads to spoilage. Our solution autonomously tracks cumulative temperature exposure, providing consumers with reliable indicators of meat freshness. This innovation promises improved food safety, better consumer health outcomes, and a win-win scenario for producers and consumers alike.

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Innovative Cell-Based Bio-Indicator to Enhance Meat Freshness Monitoring Beyond Sell-By Dates

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  1. Cell by Date Bio-Indicator for Meat Spoilage

  2. Introduction

  3. Introduction

  4. Introduction

  5. Problem with Food Industry Specification Design Modelling Implementation Testing/Validation • PROBLEM: Printed sell-by date is based on predictive challenge testing • Not reflective of product exposure to environment • Printed Sell By Date is unreliable • SOLUTION: Monitor level of exposure and report it – Tell Consumer when meat is not fit for consumption

  6. Motivations Specification Design Modelling Implementation Testing/Validation • Fresh meat is a highly perishable food • Survey studies show temperature conditions >10°C are usual during transportation, retail storage, and consumer handling. • 1/3 of Adults use spoilt food • Colouring and packaging methods are deceptive • Win-win situation for producers-consumers • Recycling of old projects – pH, temperature, BFP

  7. Specification Specification Design Modelling Implementation Testing/Validation • Report When food is ‘off’ • Report cumulative Temperature exposure of food • Temp is largest factor in spoiling • Easiest way to report if food is bad • Easy To Use • Visual Reporter – Wow ! factor • Improve Accuracy of other attempts: • SSM, Intelligent Cooling, Antimicrobial • Only Threshold devices • System must be stable for length of shelf-life • No contact with food – Need to obey HSA standards

  8. Considerations/Problems Specification Design Modelling Implementation Testing/Validation • Report Cumulative Temperature exposure of food • Need memory to do this / Integrator fct • Visual Reporter • Needs to have long degradation time • Improve Accuracy • Limited Crosstalk with chassis • Easily tuneable threshold • Stable for length of Shelf Life • HSA standards • Project must not contaminate food

  9. Solutions Specification Design Modelling Implementation Testing/Validation • Integrator • Improved Accuracy • Stability • HSA standards • Long Degradation Time • Firefly FP Cell Free System

  10. Solutions Specification Design Modelling Implementation Testing/Validation • Cell-free system • Widely-used in vitro tool used to study biological reactions • Less of the complex interactions cf.whole cell. • Provides molecular machinery that can be used in reactions with the absence of many cellular components.

  11. Solutions Specification Design Modelling Implementation Testing/Validation • Integrator • Improved Accuracy • Stability • HSA standards • CFS increases stability due to lack of degrading enzymes • CFS stable for up to 4 days • CFS agrees with HSA standards Cell Free System

  12. Luciferase RBS Te Te Pc Design Specification Design Modelling Implementation Testing/Validation Temp. Increase Cell-free extract medium O2 Luciferin LIGHT!!! Photinus luciferin + O2 + ATP <=> oxidized Photinus luciferin + CO2 + AMP + diphosphate + light

  13. Modelling Specification Design Modelling Implementation Testing/Validation • Concept of predictive microbiology • Involves knowledge of microbial growth responses to environmental factors • Expressed in quantitative terms using mathematical equations • Data used to interpret the effect of varying conditions on microbial growth. • Integrative Temp function of product • Leads to “intelligent” product management systems • Optimization of food quality and safety at the time of consumption

  14. Implementation Specification Design Modelling Implementation Testing/Validation Simplest System : Phase 1 Bio indicator Colour scheme BAD Good OK

  15. Implementation Specification Design Modelling Implementation Testing/Validation • Simplest System : Phase 2 • Discrete Change In addition Phase 1 Good Bad Eg. Discrete Change At 25oC for more than 2 hours

  16. Testing Specification Design Modelling Implementation Testing/Validation • Focus on proof of concept • Period of 24 hours as opposed to 4 days • Regulated luciferase synthesis that corresponds to predicted outcome • Light intensity as a measurement (compare with colour scheme strip)

  17. Time line • Week 3-4 – Modelling + GeneART • Week 4-6 – Experiment implementation • Week 7-8 – Testing / Validation • Week 9-10 – Polish the project + documentation

  18. Conclusion • Cell by date: • Mimics printed ‘sell by date’ with additional temperature exposure tracking • More accurate than conventional method • Will improve consumer health • Focuses on use of experimentation & proof of concept

  19. End • Questions?

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