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Tissue materials and analysis methods

This study investigates the fold increase in expression activity of BDDP (DEAT) over d35S (DAT) promoters using diverse plant materials and different reporter markers and assay methods. The analysis encompasses transient GUS expression in grapevine SE through fluorometric assays, stable GUS expression in transgenic tobacco, and transient/stable VvMybA1 expression using both color histogram-based analysis and tissue anthocyanin concentration measurement. Results highlight the efficacy of various approaches in evaluating promoter activity across different plant systems.

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Tissue materials and analysis methods

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  1. Fold Increase of expression level of BDDP (DEAT) over d35S (DAT) promoters Tissue materials and analysis methods Supplemental Figure 2. Comparison of fold increase of expression activity of BDDP (DEAT) over d35S (DAT) promoter based on various plant materials, reporter markers and assay approaches. A, Transient GUS expression in grapevine SE via fluorometric assay; B, Stable GUS expression in transgenic tobacco via fluorometric assay (both A and B were from Li et al. 2004); C, Transient VvMybA1 expression in grapevine SE via color histogram-based analysis; D and E, Stable VvMybA1 expression in in vitro leaves and sepal of transgenic tobacco via color histogram-based analysis; F, Stable VvMybA1 expression in sepal of transgenic tobacco via tissue anthocyanin concentration measurement.

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