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Dr. Mueller’s Mechanistic Enzymology Lab Chemistry and Biochemistry Department

A Day in the Lab with. Name: Ms. Marguerite McDonald Title: Biochemist, grad student Concentration: Enzymology Work Place :. Dr. Mueller’s Mechanistic Enzymology Lab Chemistry and Biochemistry Department University of Delaware Graduate Program. PROTEIN. RNA. DNA. The Central Dogma.

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Dr. Mueller’s Mechanistic Enzymology Lab Chemistry and Biochemistry Department

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  1. A Day in the Lab with... Name:Ms. Marguerite McDonaldTitle: Biochemist, grad studentConcentration: EnzymologyWorkPlace : Dr. Mueller’s Mechanistic Enzymology Lab Chemistry and Biochemistry Department University of Delaware Graduate Program

  2. PROTEIN RNA DNA The Central Dogma Biochemistry http://bioweb.wku.edu/courses/BIOL115/Wyatt/Nucleic_Acids/mRNA2.htm

  3. The study of the action (the mechanism) of an enzyme  synthase Pseudouridine synthases Mechanistic Enzymology http://www.sdnhm.org/exhibits/epidemic/teachers/images/bacteria.jpg

  4. O O H N H N N O O N The Chemistry: Isomerization of RNA

  5. TruA’s substrate: transfer RNA

  6. Purifying radioactive tRNA and TruA SDS-polyacrylamide gel showing pure TruA and TruA before purification urea-polyacrylamide gel showing pure tRNA and tRNA with DNA contamination

  7. Questions? O H H N N O

  8. H N O O O O N O O O H H N N N O N H H N N N O O

  9. H N O O O O O O N O O O H H N N N O N H H N N N O H N O N Pseudouridine Synthases

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