'Protein purification' diaporamas de présentation

Chapter 5 Proteins: Their Primary Structure and Biological Function

Chapter 5 Proteins: Their Primary Structure and Biological Function. Essential Question. What structural forms do polypeptide chains assume, how can the sequence of amino acids in a protein be determined, and what are the biological roles played by proteins?. Outline.

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CHMI 2227E Biochemistry I

CHMI 2227E Biochemistry I. Protein purification and characterization. Protein purification. Proteins are always found as part of complex mixtures; While some proteins are quite abundant (antibodies in the blood), others are found in minute amounts (a few molecules per cell);

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Principles of Shotgun Proteomics and Proteogenomics

InnoMol Proteomics Workshop April 8, 2014. Principles of Shotgun Proteomics and Proteogenomics. Boris Ma ? ek Proteome Center Tuebingen. General MS-based proteomics workflow. Aebersold R and Mann M. 2003 . Nature 422 : 198-207. Principle of protein database search.

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Purification of Green Fluorescent Protein (GFP)

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Amino Acid Analyser

2000.6.17 ??3? ???. Amino Acid Analyser. Amino Acid Analyser Products Ion exchange chromatography Application An example of application. Amino Acid Analyser . AMINO ACID ANALYSER (AAA)

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Protein Purification

Protein Purification. Protein Purification. What do you know about proteins? Why do we need to purify proteins? What are you curious about?. Bacterial chromosome. What is Transformation?. Uptake of foreign DNA, often a circular plasmid. Plasmid.

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V National Conference BIFI, Zaragoza, Febrero 2011

V National Conference BIFI, Zaragoza, Febrero 2011. OVERVIEW OF EXPERIMENTAL FACILITIES AT BIFI-BIO. OLGA ABIAN oabifra@unizar.es. V National Conference BIFI, Zaragoza, Febrero 2011. GENERAL AREAS OR ACTIVITIES PERFORM AT BIFI FACILITIES.

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Protein Analysis and Purification Analytical Separations Gel-electrophoresis IEF 2D-gels

Protein Analysis and Purification Analytical Separations Gel-electrophoresis IEF 2D-gels Preparative Separations Various chromatographic methods. Total E. coli Proteins - 2-Dimensional Gel. Overview of Protein Purification Introduction Protein Properties Types of Separations

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My Summer

My Summer. ~ ?????? ~ 891607 ???. ??? ??? Dr. Huey-Nan Wu ???. ????. D ????????????????? ????????????. C prM E NS1 NS2 NS3 NS4 NS5. Introduction (I). Dengue Virus. S.S. RNA. NS3 N-terminal Helicase NS3 C-terminal Protease

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Insight into the Regulation of Dynein by Dynactin

Insight into the Regulation of Dynein by Dynactin. Drew Calhoun Dr. Elisar Barbar Biochemistry & Biophysics Dept. Motor Proteins. THE CYTOPLASMIC DYNEIN COMPLEX. Microtubule binding domain. Heavy chain: 530 kDa, motor domain. Roadblock: (LC), 10.9 kDa, dimer. Light intermediate chain:

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Kelly Bachta , MD PhD Ramakrishnan Laboratory UW Internal Medicine Residency

Research In Progress. Towards an Understanding of the role of ESAT-6 in Mycobacterial Granuloma Formation. Kelly Bachta , MD PhD Ramakrishnan Laboratory UW Internal Medicine Residency. Why is TB so scary? Drug Resistance.

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Protein Purification

Protein Purification. Contains protein and non protein. salt. Non protein. Salt + protein. Add buffer. Buffer Solution contains: Mixture of salt and protein. Reduce the salt concentration (by dialysis). Dialysis: Reducing the salt concentration in the mixture solution. gf.

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Investigating conformation specific drug targets of human Thymidilate synthase

Investigating conformation specific drug targets of human Thymidilate synthase. Ukegbu B. Iroha Department of Pharmaceutical sciences, University of South Carolina, Columbia. Mentor: Dr. Sondra Berger December 10, 2004.

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Using and Analyzing Fluorescent Proteins: BLAST KEY

Using and Analyzing Fluorescent Proteins: BLAST KEY. 2013 Workshop C: Cloning DNA to Make Proteins Dina N Kovarik, MS, PhD Digital World Biology. Research Questions.

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Biochemistry Lab

Biochemistry Lab. Protein Purification - 2 Chromatography. Methods of Cell Lysis. Passive Methods: Freeze-thaw Chemical Enzymatic Osmotic Pressure. Physical Methods: Sonication Cell Bomb Homogenizer. Additional Considerations:. Volume Cell Type Toxicity Cost.

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Using BLAST to Analyze Proteins Part 2: Comparing Fluorescent Proteins

Using BLAST to Analyze Proteins Part 2: Comparing Fluorescent Proteins. 2013 Workshop C: Cloning DNA to Make Proteins Dina N Kovarik, MS, PhD NWABR. Fluorescent Proteins are Valuable Tools. Locate proteins in the cell Track the migration of cells Reporter of expression.

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Experimental Application of Mathematical Models

Experimental Application of Mathematical Models. Christopher Smith. Microtubules Kinesins / molecular motors Kinetochores Cytokinesis Microscope development. What I do. Anaphase in mitosis. Kinetochore- microtubules. CENP-Q. What I do. Watch this space. Protein Purification.

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Center for Integrated Animal Genomics

Research Experience in Molecular Biotechnology & Genomics Summer 2008. Center for Integrated Animal Genomics. Jessian Resto 1 ; Kristen Johansen 2 ; Jorgen Johansen 2 ; Laurence Woodruff 2 ; Xiaomin Bao 2 ; Hui Deng 2 ; Yun Ding 2 ; Weili Cai 2 ; Changfu Yao 2

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