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Using and Analyzing Fluorescent Proteins: BLAST KEY

Using and Analyzing Fluorescent Proteins: BLAST KEY. 2013 Workshop C: Cloning DNA to Make Proteins Dina N Kovarik, MS, PhD Digital World Biology. Research Questions.

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Using and Analyzing Fluorescent Proteins: BLAST KEY

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  1. Using and Analyzing Fluorescent Proteins: BLAST KEY 2013 Workshop C: Cloning DNA to Make Proteins Dina N Kovarik, MS, PhD Digital World Biology

  2. Research Questions The cloning and protein purification experiments you have been conducting in the laboratory involve mTomato, also called red fluorescent protein (RFP). • Is red fluorescent protein (RFP) related to its famous cousin, GFP? • What other fluorescent proteins, if any, are closely related to GFP and/or RFP?

  3. Comparing eGFP and pLemon-YFP DNA Sequences

  4. Comparing RFP and mGrape-PFP DNA Sequences

  5. Comparing eGFP and RFP DNA Sequences

  6. Comparing eGFP and pLemon-YFP Protein Sequences

  7. Comparing RFP and mGrape-PFP Protein Sequences

  8. Comparing All Fluorescent Proteins:BLAST Scores

  9. All Fluorescent Proteins: Distance Tree of Results

  10. Diversity and Key Mutations

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