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This study explores the various configurations of the SV40 promoter and 3’ UTR, assessing their effects on luciferase expression. By analyzing sequences such as Wt (+1/+2743), and other segment combinations like (+1/+937) and (+1/+1015), we delve into the regulatory mechanisms at play. This research lays the groundwork for understanding promoter functionality and post-transcriptional regulation in gene expression studies. Findings could have implications in genetic engineering and therapeutic applications.
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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 SV40 promoter Luciferase 3’-UTR Wt (+1/+2743) (+1/+937 - +1015/+2743) (+1/+2518) (+1/+937 - +1015/+2518) (+1/+1325) (+1075/+2518) (+1/+820) (+1/+313) (+859/+1171) (+859/+937 - +1015/+1171) (+859/+1010) (+859/+983) (+859/+954) (+859/+932) (+896/+1010) (+933/+1171) (+956/+1171) (+984/+1171) (+1011/+1171) (+933/+1030) (+933/+1010)