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Cytotoxic Effects of Subamolides from Cinnamomum subavenium on Human Colorectal Cancer Cells

This study explores the chemical constituents and biological activity of Cinnamomum subavenium leaves, focusing on subamolides D and E. Utilizing propidium iodide staining and cytometry analysis, we evaluated their ability to inhibit cell cycle progression in SW480 colorectal cancer cells. Results indicate that these subamolides induce apoptosis and cause DNA damage in a concentration- and time-dependent manner, leading to increased sub-G1 apoptotic fractions. This research is the first to detail the effects of these compounds on cell cycle phases, highlighting their potential chemopreventive properties.

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Cytotoxic Effects of Subamolides from Cinnamomum subavenium on Human Colorectal Cancer Cells

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  1. Kuo, Soong-Yu IUFRO Division 5 Conference 5.07B Using Plantation And Small-Diameter Timber In Composites

  2. Soong-Yu Kuo1, Wen-Li Lo1, Chung-Yi Chen1, Jin-Cherng Huang2 and Hsien-Toung Huang2 Chemical and Cytotoxic Constituents from the Leaves of Cinnamomum subavenium 1Basic Medical Science Education Center, Fooyin University; Kaohsiung County 83101, Taiwan (R.O.C.); 2Department of Forest Products Science, National Chia-Yi university, Chiayi, Taiwan (R.O.C.)

  3. Aim of Work And Methods • Motive：The chemical constituents and the biological activity of the leaves of Cinnamomum subavenium Miq. (Lauraceae) have not yet been reported. • Purpose：To elucidate the cytotoxic mechanism of subamolides (1 and 2), the capacity of subamolide D (1) and subamolide E (2) to inhibit cell cycle phase distribution. • Method：Propidium iodide staining and cytometry analysis were used to evaluate the cell cycle progression of the treated SW480 cells.

  4. Results • The marked increase in sub-G1 apoptotic fraction appeared in cells treated with 25-100 μM subamolide D (1) or subamolide E (2) in a concentration-dependent manner. • Additionally, incubation with 25, 50 and 75 μM subamolide D (1) for 24 h resulted in the accumulation of cells in G2/M phase (25.4, 29.8 and 23.0 %, respectively) compared with that in control (15.4 %). • When cells were treated with 100 μM subamolide D (1) or subamolide E (2), > 25 %, > 78 %, and > 85 % sub-G1 fraction was noted for treated cells after 8 h, 16 h, and 24 h exposure, respectively.

  5. Conclusion • Subamolides D (1) and E (2) caused DNA damage in a dose- and time-dependent manners. • These findings suggest that compounds present in C. subavenium Miq. (e.g. subamolides D (1) and E (2)) may have chemopreventive properties. • This study is the first to investigate the effect of subamolides D (1) and E (2) on cell cycle phase distribution in human colorectal cancer cells.

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