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Comparative Analysis of pH Effects on Arabidopsis CPLL Eluates and Crude Extract Protein Content

This study investigates the impact of pH on protein content in Arabidopsis crude extracts and CPLL eluates. By comparing a control crude extract at pH 7 with eluates from CPLL beads at pH 4, 7, and 9, we observe a substantial overestimation of the CPLL-specific effect. The combined influence of pH and CPLL extraction methods results in a 16% loss (152 proteins) and a remarkable 71% gain (659 proteins) compared to the initial crude extract, which contains a total of 932 proteins. This underscores the importance of pH in protein extraction methods.

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Comparative Analysis of pH Effects on Arabidopsis CPLL Eluates and Crude Extract Protein Content

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  1. Arabidopsis Crude extract pH 7 Arabidopsis CPLL eluate pH all 152 780 659 Supplemental Figure 2. Comparison of only one control crude extract (pH 7) with eluates from CPLL beads applied at pH 4, 7 and 9 leads to overestimation of the CPLL-specific effect. In this case the combined effects of pH and CPLL would cause a “loss” of 152 (16 %) and a “gain” of 659 (71 %) proteins in comparison to the crude extract (932 proteins, 100 %).

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