1. DNA Fingerprinting Procedure & Applications

2. What is a DNA Fingerprint? • A pattern of bands that is unique to each person (unless you have an identical twin)

3. How is a DNA Fingerprint Made?

4. Interpreting DNA Fingerprints • Restriction enzymes cut at certain DNA sequences. • Because everyone’s sequence is different, the enzymes will cut in different places, producing different sized fragments. • The smaller the fragment, the further it will move in the gel. • Thicker bands mean that there are many fragments that size. • The more bands two people have in common, the more closely related they are.

5. Applications of Fingerprinting • Identifying criminals – compare DNA sample from a crime scene to suspect’s DNA These fingerprints compare two DNA samples found at a crime scene Which suspect is guilty? Where did the other DNA at the crime scene come from?

6. Applications of Fingerprinting • Fingerprinting may also be used to link parents to their children (ie. paternity testing) • This can be more complicated because children will not be an exact match to either parent; they will have some bands from each parent You are NOT the father!

7. Article: DNA barcoding • What is iBOL? • What are the proposed benefits of this technology? • Note: Pay special attention to how the reader is “hooked” in the first paragraph.