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This study compares two lots of UHRR-Cy3 and UHRR-Cy5 for self-hybridization, showing high correlation in Cy3 and Cy5 intensity backgrounds with log scaling. The correlation coefficients for Cy3 and Cy5 are 0.9925 and 0.9822 respectively.
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A B Lot -to-lot comparison Lot#1 UHRR-Cy3/ lot#2 UHRR-Cy5 Self -to-self hybridization lot#1 UHRR-Cy3/ lot#1UHRR-Cy5 R = 0.9925 R = 0.9822 Cy5 Intensity-Bkgr, Log Scaling Cy5 Intensity-Bkgr, Log Scaling Cy3 Intensity-Bkgr, Log Scaling Cy3 Intensity-Bkgr, Log Scaling