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Molecular Typing of Salmonella enterica Serovars using Two PFGE Systems

Molecular Typing of Salmonella enterica Serovars using Two PFGE Systems. Geraldine Doran Martin Cormican. Interim Salmonella Reference Laboratory Ireland. Molecular Typing of S.enterica Serovars using two PFGE Systems. Serovars. S. Kedougou S. Bredeney.

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Molecular Typing of Salmonella enterica Serovars using Two PFGE Systems

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  1. Molecular Typing of Salmonella enterica Serovars using Two PFGE Systems Geraldine Doran Martin Cormican Interim Salmonella Reference Laboratory Ireland

  2. Molecular Typing of S.enterica Serovars using two PFGE Systems • Serovars S. Kedougou S. Bredeney • SystemsPharmacia Gene Navigator • Bio-Rad CHEF Mapper (PulseNet)

  3. S. Kedougou Results • Pharmacia: 4 Subtypes • CHEF Mapper: 7 Subtypes

  4. Molecular Typing of S.enterica Serovars using two PFGE Systems Comparison of 2 Systems • TimePharmacia 6 Days • CHEF Mapper 4 Days (3) • Reproducibility Both reproducible • Result QualityCHEF Mapper Easier to intrepret • Standardised

  5. PFGE of S. Kedougou using Pharmacia λ A1 A1 A2 λ A3 A3 A3 A4 λ ? A4 A4 A1 λ kb 339 291 242 194 145 97.0 48.5

  6. PFGE of S. Kedougou using CHEF Mapper λ A1 A6 A2 λ A3 A3 A5 A4 λ A5 A5 A7 A1 λ kb 339 291 242 194 145 97 48

  7. PFGE of S. Kedougou CHEF Mapper Pharmacia kb Λ A1 A1 A2 λ A3 A3 A3 A4 λ A4 A4 A4 A1 λ λ A1 A1 A2 λ A3 A3 A3 A4 λ A4 A4 A4 A1 λ 339 242 145 97 48

  8. S Kedougou Pharmacia Reproducibility Run 1 Run 2 λ A1 A1 A2 λ A3 A3 A3 A4 λ ? A4 A4 A1 λ λ A1 A1 A2 λ A3 A3 A3 A4 λ A4 A4 A4 A1 λ kb 339 291 242 194 145 97 48

  9. PFGE of S. Bredeney CHEF Mapper Pharmacia λ A A2 A3 λ B C D E λ F G λ A A2 A3 B C D E F G λ kb 291 242 194 145 97 48

  10. Dendogram of S. Kedougou using Bionumerics Software

  11. Conclusions CHEF Mapper Quicker Easier to Intrepret More Reproducible Standardised (PulseNet)

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