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This document presents a detailed protocol for utilizing PFGE (Pulsed-Field Gel Electrophoresis) with the CeuI restriction enzyme and 16S rRNA probes to analyze microbial genomes. It includes steps for sample preparation, digestion conditions, gel electrophoresis, and analysis of results. This methodology helps refine microbial identification and taxonomy, facilitating deeper insights into microbial diversity and ecology. Ideal for researchers in microbiology and molecular biology seeking to enhance their genomic analysis techniques.
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