p histone h3

1. Rabbit Anti-Human Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Polyclonal Antibody CABT-LM00257 Rabbit(HIST1H3A) Lot. No. (See product label) PRODUCT INFORMATION Rabbit Polyclonal Antibody to Human Acetyl- and Phospho-Histone H3 (Lys9/Ser10) molecule Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase. Acetyl- and Phospho- Histone H3 (Lys9/Ser10) Antibody detects endogenous levels of histone H3 only when modified both by acetylation at lysine 9 and phosphorylation at serine 10. Acetyl- and Phospho-Histone H3 (Lys9/Ser10) a synthetic acetylated and phosphorylated peptide corresponding to residues surrounding Lys9 and Ser10 of human histone H3 Rabbit Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated and phosphorylated peptide corresponding to residues surrounding Lys9 and Ser10 of human histone H3. Antibodies are purified by protein A and peptide affinity chromatography. Human Mouse,Rat N/A WB,IHC WB: 1:1000 IHC-P: 1:50 Endogenous 17 Product Overview Antigen Description Specificity Target Immunogen Host Source Species Cross Reactivity Conjugation Applications Dilution Sensitivity Molecular Weight PACKAGING Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20 degree Celsius. This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. Storage Warning ANTIGEN GENE INFORMATION Creative Diagnostics. All rights reserved 45-16 Ramsey Road Shirley, NY 11967, USA Tel: 631-624-4882 ·Fax:631-614-7828 E-mail: info@creative-diagnostics.com www.creative-diagnostics.com

2. Gene Name HIST1H3A histone cluster 1, H3a [ Homo sapiens (human) ] HIST1H3A HIST1H3A; histone cluster 1, H3a; H3 histone family, member A , H3FA, histone 1, H3a; histone H3.1; H3/A; histone H3/a; histone H3/b; histone H3/c; histone H3/d; histone H3/f; histone H3/h; histone H3/i; histone H3/j; histone H3/k; histone H3/l; histone 1, H3a; H3 histone family, member A; H3FA OfficialSymbol Synonyms GeneID 8350 mRNA Refseq NM_003529.2 Protein Refseq NP_003520.1 MIM 602810 P68431 UniProt ID Chromosome Location 6p22.1 Alcoholism; Amyloids; Disease; Factors involved in megakaryocyte development and platelet production; Gene Expression; Hemostasis; Meiosis; Pathway REFERENCES 1.Breaking the HAC Barrier: histone H3K9 acetyl/methyl balance regulates CENP-A assembly. Ohzeki J, et al. EMBO J, 2012 May 16. 2.Plant homeodomain (PHD) fingers of CHD4 are histone H3-binding modules with preference for unmodified H3K4 and methylated H3K9. Mansfield RE, et al. J Biol Chem, 2011 Apr 1. 3.A chemical method for labeling lysine methyltransferase substrates. Binda O, et al. Chembiochem, 2011 Jan 24. Creative Diagnostics. All rights reserved 45-16 Ramsey Road Shirley, NY 11967, USA Tel: 631-624-4882 ·Fax:631-614-7828 E-mail: info@creative-diagnostics.com www.creative-diagnostics.com