HMTs Screening HMTs Screening Creative Biogene offers biochemical assays for a wide range of HMT enzyme targets, including DOT1, EZH1, EZH2, G9a, NSD1, NSD2, PRMT1, PRMT3, PRMT4, PRMT5, PRMT6, PRMT8, SET1B, SET7/9, SET8, MLL-1, SMYD3, SUV39H1, SUV39H2, and so on. HMTs assays in Creative Biogene utilize highly specific antibodies that recognize methyl groups. Generally, there are three steps in our assays. First, S- adenosylmethionine (SAM) is incubated with a sample containing assay buffer and purified recombinant methyltransferase enzyme. Second, acceptor beads, a primary antibody, and then donor beads are added. The third step is to read the Alpha-counts.
Brief Introduction to Methyltransferases Brief Introduction to Methyltransferases Methyltransferases are enzymes which transfer a methyl group from SAM to their substrates. DNA methyltransferases modify DNA by transferring a methyl group to cytosines. DNA methylation process suppresses gene expression, which is critical for transcriptional silencing during imprinting, X-inactivation and other developmental processes. In addition, DNA methylation plays a key role in the development of nearly all types of cancer.