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This study investigates the induction of AI-2 activity in the presence of ribulose-5-phosphate (Rib-5-P) and its interaction with various enzyme buffers. The analysis highlights the effect of Rib-5-P on biochemical pathways and enzyme activity over time. Measurements are taken to understand the relationship between enzyme activation and the presence of ribulose-5-phosphate under controlled conditions. Insights from this research may contribute to the understanding of metabolic regulation and intercellular signaling.
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104 A 104 103 103 102 AI-2 activity [fold induction] 102 101 101 100 100 10-1 10-1 Control MHF Rib-5-P Xyl-5-P Rul-5-P B AI-2 activity [fold induction] 200 C Buffer Buffer + Rib-5-P Buffer + enzyme Buffer + Rib-5-P + enzyme Buffer + Rib-5-P + enzyme (inactive) 150 AI-2 activity [fold induction] 100 50 0 0 1 2 3 4 5 6 24 Time [h]