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dengue virus

dengue virus. WHO: 50 - 100 million cases/yr. Sharon Isern, PhD Yancey Hrobowski, PhD Joshua Costin, PhD Kelli Barr, PhD Krystal Fontaine Craig Rees Cindo Nicholson Robert Garry, PhD - Tulane University Ram Samudrala, PhD - University of Washington Tom Monath, PhD - Acambis

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dengue virus

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  1. dengue virus WHO: 50 - 100 million cases/yr

  2. Sharon Isern, PhD Yancey Hrobowski, PhD Joshua Costin, PhD Kelli Barr, PhD Krystal Fontaine Craig Rees Cindo Nicholson Robert Garry, PhD - Tulane University Ram Samudrala, PhD - University of Washington Tom Monath, PhD - Acambis Michael Holbrook, PhD - UTX, Galveston Elizabeth Hunsperger, PhD - CDC, San Juan ONR /DTRA /US Army / NIH Acknowledgements

  3. Kaufmann, et al. PNAS 103:12400-12404 (2006)

  4. Zhang, et al. Nature Structural Biology 10:907 (2003)

  5. Development of novel virus entry inhibitors virus host cell inhibitor entry blocked

  6. HOW DO YOU DEVELOP A VIRAL INHIBITOR?

  7. Dengue Plaque Assay control control peptide 57IIb peptide 80FP peptide 81IIb peptide 59PA

  8. DON’T GET FOOLED!

  9. No Toxicity in MTT Assay

  10. Effect of Scrambled Amino Acid Order

  11. Effect of dengue inhibitory peptides on Sindbis virus infectivity

  12. DENV-2 MAILGDTAWDFGSLGGVFTSIGKALHQVFGAIY DENV-1 -------------I------V--LI--I--TA- DENV-3 -------------V---LN-L--MV--I--SA- DENV-4 -----E-------V---L--L---V-----SV- WNV L-A----------V----------V-----GAF YFV L-VM--V----S-A--F------GI-T---SAF SLEV L-V----------I------I---------GAF JEV L-A----------I----N-I---V-----GAF RSSEV LTVI-EH------T--FL--------T-L-GAF CEEV LTVI-EH------A--FLS-I-----T-L-GAF

  13. HOW DO THESE THINGS WORK?

  14. Next Steps?

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