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Protein Sequencing Using MALDI-TOF-TOF Mass Spectrometry: A Case Study

Explore a detailed example of protein sequencing using MALDI-TOF-TOF mass spectrometer. Learn about MS/MS spectra interpretation and sequence matching in canine troponin T.

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Protein Sequencing Using MALDI-TOF-TOF Mass Spectrometry: A Case Study

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  1. Yergey et al.(2002)

  2. An example of protein sequencing using a MALDI-TOF-TOF mass spectrometer. The MS/MS spectrum recorded from the fragmentation of an ion at 1016.47. The interpretation of this spectrum gave the putative sequence VDFDDXHR, where X denotes either an L or an I. This sequence is matched to VDFDDIHR in canine troponin T.

  3. CHUNG et al. (2006)

  4. Structure elucidation of an arabinoxylan oligosaccharide by normal-phase chromatography-MALDI-TOF/TOF tandem mass spectrometry (Maslen et al. 2007, Carbohydrate Research, 342, 724-735).

  5. OBRIGADO PELA ATENÇÃO!!!!

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