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This supplementary figure presents a comprehensive analysis of RNA1 and RNA2 interactions, highlighting the use of various restriction enzymes including BlnI, SacI, and SmaI for cloning purposes. The data showcases the effective incorporation of the aadA resistance gene into the pBpless-Tx vector, illustrating the molecular strategies employed in the research. Additionally, the endpoints of RNA segments are specified, emphasizing precise genetic manipulations that enhance understanding of RNA functionalities in cellular contexts.
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Supplementary Fig.1 b a 8 7 RNA1 RNA1 43 44 114 47 21 10 9 22 RNA2 RNA2 49 50 115 53 62 63 c 369 370 369 370 Bln I- - Sac I Bln I- - blunt A B E D 371 372 371 222 372 369 222 369 Bln I- Bln I- - blunt - Sac I F C Bln I / Sac I Bln I / Sma I Bam HI Sma I Hin dIII Bln I SacI CapAt G Prrn pBpless-Tx aadA SpecR