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Identification of ERGIC3 as a Novel Gene Associated with Lung Adenocarcinoma

This study investigates the role of ERGIC3, a newly identified gene linked to lung adenocarcinoma. Utilizing suppression subtractive hybridization and cDNA microarray techniques, we analyzed gene expression in lung cancer samples, revealing significant upregulation of ERGIC3. With an 88.57% positivity rate in immunohistochemical evaluations, ERGIC3 demonstrates potential as a biomarker for early diagnosis and therapeutic targets. This research highlights the importance of understanding genetic mechanisms in lung cancer and positions ERGIC3 as a candidate oncogene.

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Identification of ERGIC3 as a Novel Gene Associated with Lung Adenocarcinoma

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  1. Identification of differentially expressed genes in human lung adenocarcinoma: ERGIC3 as a novel lung cancer-related gene Mingsong Wu Department of Cell Biology and Genetics， Zunyi Medical University，China Las Vegas 2014.10

  2. Part I 1、Background 1.1 epidemiology of lung cancer

  3. Mortality in lung cancer in selected country, 1950－2006 Jemal et al.Cancer Epidemiology, Biomarkers & Prevention. (2010)

  4. Subtype of lung cancer small-cell lung carcinoma lung cancer squamous cell carcinoma non-small cell lung carcinoma large-cell carcinoma adenocarcinoma

  5. New tumor marker of lung cancer for early diagnosis and treatment To investigate genetic mechanism and new target

  6. 1.2 Molecular basis of LC

  7. 1.3 Suppression subtractive hybridization(SSH) • cDNA Microarray • mRNA Differential • display，DD • Representational diff- • erence analysis，RDA • Suppression subtractive • hybridization, SSH lower false positive,higher specific &sensitive

  8. 2、Results 2.1 Construction of AC-associated libray 2.1.1 Total RNA & mRNA total RNA mRNA

  9. 2.1.2 Analysis of Rsa I digestion digestion of double cDNA

  10. 2.1.3 Ligation efficiency analysis 35％

  11. 2.1.4 Supression PCR

  12. 2.2 Clone of EST 2nd PCR Product pGEM T-easy Vector White clones PCR Digestion Sequence FSL：485 up-regulated RSL：172 down-regulated Analysis

  13. 2.3 Bioinformatic analysis

  14. 2.3.1 BLAST Gene：177（new：152） Unknown EST: 44 Gene：59（new：54） Unknown EST: 10

  15. 2.3.2 GO Ontology

  16. 2.3.3 Biocatar pathway RSL

  17. 2.3.4 Biocatar pathway FSL

  18. 2.4 Sreen of SSH-libraries Higher quality Higher efficiency

  19. Part II 1、Background • (Endoplasmic reticulum-Golgi intermediate compartment 3，ERGIC3) • Cycles between the endoplasmic reticulum and Golgi • II type transmembrane protein, 383 aa, 43.2 kDa

  20. Herzog， et al. 2006

  21. Mutation: COPII ↓ 25％Accumulation of protein and lipid in ER • Inhibits ER stress-induced cell death • FUNCTION? • TUMOR MARKER

  22. 2、RESULTS 2.1 Expression of ERGIC3 in NSCLC 2.1.1 mRNA of ERGIC3

  23. 2.1.2 Protein of ERGIC3

  24. 2.1.3 Protein of ERGIC3

  25. Evaluation of immunohistochemical positivity in lung cancer tissues Positive rate:88.57% AC>SCC Score: −, no staining; +, <30%; ++, 31–60%; +++, >60% positive cells

  26. 2.1.4 Location of ERGIC3

  27. 2.1.4 Location of ERGIC3

  28. 2.2 Functions of ERGIC3 in lung cancer 2.2.1 ERGIC3 promotes cellular growth

  29. 2.2.2 ERGIC3 promotes cellular migration

  30. 2.2.3 Stable overexpression of ERGIC3 promotes cellular migration

  31. 3、Summary • Library of differentially expressed genes in lung cancer. • Potential targets for diagnostic & therapeutic usage. • Two lung cancer associated genes. • ERGIC3 may be a new biomarker for lung cancer. • ERGIC3 may be a novel candidate oncogen for lung cancer.

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