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The Jackson Laboratory Bar Harbor, ME

Genetic tools and resources for orofacial clefting research. PIs: Leah Rae Donahue, Ph.D. Stephen Murray, Ph.D. Co-Investigators: Tom Gridley, Ph.D. The Jackson Laboratory Bar Harbor, ME. FaceBase Mouse Repository at JAX importation, cryopreservation, GQC, distribution.

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The Jackson Laboratory Bar Harbor, ME

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  1. Genetic tools and resources for orofacialclefting research PIs: Leah Rae Donahue, Ph.D. Stephen Murray, Ph.D. Co-Investigators: Tom Gridley, Ph.D. The Jackson LaboratoryBar Harbor, ME

  2. FaceBase Mouse Repository at JAX importation, cryopreservation, GQC, distribution • ~ 50 strains in the JAX FaceBase Repository • most are public, some still private, some imported and under development for distribution • new knockout strains - most on B6;129 background • Bambi, Osr1, Osr2, Jag2, Snai1 and Snai2, Gad1, Slc32a1, Tgfbr2, Wnt9b • new Cre strains • Osr2 (B6.129S1), Tgfb3 (STOCK) • new ENU induced mutant strain (on FVB.A) • Irf6 Genetic Resource Science

  3. FaceBaseCre Driver Project:Current progress and future plans • Goal: To generate 15 Cre driver strains for orofacialclefting research • Initial plan is to make four BAC transgenics (Krt6-Cre, Tbx22-CreERT2, dNp63-CreERT2, Lhx7/8-Cre) and three enhancer (Visel project) CreERT2 lines (R26 knock-in). • Current progress: • All basic cassettes (CreIRESmCherry, ROSA targeting vector, etc) are complete • 2 BAC Cres (Tbx22 and p63) are cloned, Krt6 is nearly done (unique challenges). Injections to be scheduled • 2 enhancer elements are ready for targeting into JM8A3 ES cells (this month) • Future enhancer cloning will be very simple and efficient • On track to have first 7 Cre lines made by 9/2010 and characterized by 12/2010 • Future plans • Continue to develop approach as better technology emerges (e.g. 3’ UTR knock-in): proof of principle using same promoter? • Choose new drivers from data generated in other FaceBase projects and in consultation with mouse subcommittee members

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