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ENZYME ACTIVITY

ENZYME ACTIVITY. How to interpret enzyme graphs. (NEXT SLIDE). Reading Graphs. Which graph shows the amount of substrate going to zero faster?. Substrate. Substrate. Time (sec). Time (sec). TRY AGAIN. So…. Nope , the amount of substrate gets to zero in less time in the OTHER graph.

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ENZYME ACTIVITY

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  1. ENZYME ACTIVITY How to interpret enzyme graphs (NEXT SLIDE)

  2. Reading Graphs • Which graph shows the amount of substrate going to zero faster? Substrate Substrate Time (sec) Time (sec)

  3. TRY AGAIN So… • Nope, the amount of substrate gets to zero in less time in the OTHER graph. Substrate Substrate Time (sec) Time (sec)

  4. RIGHT!! So… • YES!! The amount of substrate gets to zero in less time in this graph. Substrate Substrate Time (sec) Time (sec)

  5. So… • Which enzyme works FASTER? Substrate Substrate Time (sec) Time (sec)

  6. Substrate Time (sec) TRY AGAIN So… • Nope, not this one. The faster the enzyme works, the faster the substrate will decrease. Substrate Time (sec)

  7. Substrate Time (sec) RIGHT!! So… • YES!! This enzyme works faster because the substrate decreases faster!! Substrate Time (sec)

  8. What do you think can affect the speed of an enzyme? TEMPERATURE pH AMOUNT OF LIGHT

  9. What do you think can affect the speed of an enzyme? TEMPERATURE • RIGHT!! Changing the pH of the reaction can affect the speed of an enzyme. • ANYTHING ELSE? pH AMOUNT OF LIGHT

  10. What do you think can affect the speed of an enzyme? TEMPERATURE • RIGHT!! Changing the temperature of the reaction can ALSO affect the speed of an enzyme. pH AMOUNT OF LIGHT

  11. What do you think can affect the speed of an enzyme? TEMPERATURE • RIGHT!! Changing the temperature of the reaction can affect the speed of an enzyme. • ANYTHING ELSE? pH AMOUNT OF LIGHT

  12. What do you think can affect the speed of an enzyme? TEMPERATURE • RIGHT!! Changing the pH of the reaction can ALSO affect the speed of an enzyme. pH AMOUNT OF LIGHT

  13. What do you think can affect the speed of an enzyme? TEMPERATURE • Sorry, most enzymes are NOT affected by the amount of light… • Pick again pH AMOUNT OF LIGHT

  14. Factors affecting enzyme speed • We can make a graph of enzyme speed at different temperatures.

  15. Graph of ENZYME SPEED What should go on the X axis? Speed of enzyme Temperature (°C)

  16. TRY AGAIN Graph of ENZYME SPEED Nope, the speed of the enzyme is what we are MEASURING,it should go on the Y axis. Speed of enzyme Temperature (°C)

  17. 0 10 20 50 40 30 60 Graph of ENZYME SPEED Amount of substrate What should go on the Y axis? (HINT: This is what we are MEASURING) Speed of enzyme RIGHT! Temperature should go on the X axis. Temperature (°C)

  18. 0 10 20 50 40 30 60 TRY AGAIN Graph of ENZYME SPEED Amount of substrate • What should go on the Y axis? • (HINT: This is what we are • MEASURING) • We’re not measuring the amount of substrate this time. Speed of enzyme Temperature (°C)

  19. 0 10 20 50 40 30 60 Graph of ENZYME SPEED FAST RIGHT!! The speed of enzyme should go on the Y axis. Speed of enzyme REMEMBER!! The enzyme is working FASTER As you go UP the Y axis. SLOW Temperature (°C)

  20. 0 10 20 50 40 30 60 Let’s GRAPH!! FAST Amylase is found in saliva. Click on the red button that is approximately at body temperature Speed of enzyme SLOW Temperature (°C)

  21. 0 10 20 50 40 30 60 TRY AGAIN Let’s GRAPH!! FAST Amylase is found in saliva. 5°C is close to freezing. TOO COLD!! Speed of enzyme SLOW Temperature (°C)

  22. 0 10 20 50 40 30 60 TRY AGAIN Let’s GRAPH!! FAST Amylase is found in saliva. 22°C is close to room temperature. TOO COLD!! Speed of enzyme SLOW Temperature (°C)

  23. 0 10 20 50 40 30 60 TRY AGAIN Let’s GRAPH!! FAST Amylase is found in saliva. 55°C is 131°F. TOO HOT!! Speed of enzyme SLOW Temperature (°C)

  24. 0 10 20 50 40 30 60 Let’s GRAPH!! FAST Amylase is found in saliva. 37°C is body temperature. RIGHT!! Amylase will work best at about 37°C because it is normally found in your mouth Speed of enzyme SLOW Temperature (°C)

  25. 0 10 20 50 40 30 60 Let’s GRAPH!! FAST Amylase works slowly At very low temperatures Because it is not close To body temperature Speed of enzyme SLOW Temperature (°C)

  26. 0 10 20 50 40 30 60 Let’s GRAPH!! FAST Amylase will work Faster as it gets Closer to body Temperature. Speed of enzyme SLOW Temperature (°C)

  27. 0 10 20 50 40 30 60 Let’s GRAPH!! FAST Amylase will work fastest at body Temperature. Speed of enzyme The temperature where Amylase works BEST is called the OPTIMUM TEMPERATURE SLOW Temperature (°C)

  28. 0 10 20 50 40 30 60 Let’s GRAPH!! As the temperature gets too hot, Amylase doesn’t work as well. FAST Speed of enzyme SLOW Temperature (°C)

  29. 0 10 20 50 40 30 60 Let’s GRAPH!! Click on the OPTIMUM TEMPERATURE FAST Speed of enzyme SLOW Temperature (°C)

  30. 0 10 20 50 40 30 60 Let’s GRAPH!! Click on the OPTIMUM TEMPERATURE FAST Speed of enzyme RIGHT!! SLOW Temperature (°C)

  31. FAST Speed of enzyme SLOW 0 10 20 50 40 30 60 Temperature (°C) What is the optimum temperature for this enzyme? 10°C 20°C 30°C

  32. FAST Speed of enzyme SLOW 0 10 20 50 40 30 60 Temperature (C) TRY AGAIN What is the optimum temperature for this enzyme? REMEMBER: The OPTIMUM is the temperature the enzyme works fastest at. 10°C 20°C 30°C

  33. FAST Speed of enzyme SLOW 0 10 20 50 40 30 60 Temperature (°C) TRY AGAIN What is the optimum temperature for this enzyme? REMEMBER: The OPTIMUM is the temperature the enzyme works fastest at. 10°C 20°C 30°C

  34. Right!! RIGHT!!

  35. 0 2 4 10 8 6 12 What is the optimum pH for this enzyme? FAST 9 Speed of enzyme 10 11 SLOW pH

  36. 0 2 4 6 8 10 12 TRY AGAIN What is the optimum pH for this enzyme? FAST 9 Look closely… Speed of enzyme 10 11 SLOW pH

  37. 0 2 4 6 8 10 12 TRY AGAIN What is the optimum pH for this enzyme? FAST 9 Look closely… Speed of enzyme 10 11 SLOW pH

  38. Right!! RIGHT!!

  39. 20 30 40 70 60 50 80 What is the optimum temperature for this enzyme? FAST 20°C Speed of enzyme 63°C 48°C SLOW Temperature (°C)

  40. 20 30 40 70 60 50 80 TRY AGAIN What is the optimum temperature for this enzyme? FAST 20°C Speed of enzyme 63°C 48°C SLOW Temperature (°C)

  41. 20 30 40 70 60 50 80 TRY AGAIN What is the optimum temperature for this enzyme? FAST 20°C Speed of enzyme 63°C 48°C SLOW Temperature (°C)

  42. Right!! RIGHT!! You rock at this...

  43. Temperature = 25°C Temperature = 30°C Temperature = 35°C Substrate Substrate Substrate Time Time Time What temperature does the enzyme work best at? At 25°C At 30°C At 35°C

  44. Temperature = 25°C Temperature = 30°C Temperature = 35°C Substrate Substrate Substrate Time Time Time REMEMBER: the faster the substrate decreases, the faster the enzyme is working At 25°C At 30°C At 35°C

  45. Temperature = 25°C Temperature = 30°C Temperature = 35°C Substrate Substrate Substrate Time Time Time REMEMBER: the faster the substrate decreases, the faster the enzyme is working At 25°C At 30°C At 35°C

  46. TO SHOW HOW WELL YOU UNDERSTOOD, COMPLETE THIS SHORT QUIZ RIGHT!!

  47. QUIZ - QUESTION 1 • Where in your body is the amylase enzyme found? SALIVA LIVER STOMACH

  48. TRY AGAIN QUIZ - QUESTION 1 • Where in your body is the amylase enzyme found? SALIVA LIVER STOMACH

  49. TRY AGAIN QUIZ - QUESTION 1 • Where in your body is the amylase enzyme found? SALIVA LIVER STOMACH

  50. Right!! RIGHT!!

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