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Our Test Organism

Our Test Organism. Drosophila simulans. Trait of Interest. Red vs. White Red = advantageous “mutation” Why are red eyes advantageous? Sensory perception White eyes = reduced mate tracking. Drosophila chromosome. What are we going to look at?. Two markers on X-chromosome

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Our Test Organism

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  1. Our Test Organism Drosophila simulans

  2. Trait of Interest Red vs. White Red = advantageous “mutation” Why are red eyes advantageous? Sensory perception White eyes = reduced mate tracking

  3. Drosophila chromosome What are we going to look at? • Two markers on X-chromosome • One close to the gene for white eyes • One far from the gene for white eyes • Why are we going to look at 2 markers? Red / White eye color gene 22 million bases of DNA 22 million bases of DNA “Near” “Far”

  4. Microsatellites & Genetic Markers Sequence differences (aka variation) between alleles Usually base pair repeats, insertions, or deletions Used for between & within-species comparisons

  5. Before sweep After sweep Selective Sweep/Hitchhiking This is one chromosome from 12 different people. The different colors represent different alleles for that gene. What happened?

  6. How are we going to observe & measure genetic variation?

  7. Step 3: Introduce “advantageous mutation” Allow mating for several generations

  8. Anesthetizing fliesFlyNap – Stinky!

  9. White male Red female White female Red male Sexing your flies: males have a “black butt”, females have large white abdomen

  10. How to tell males from females • Males have black abdomens • Look close at the tip of the male abdomen and you will see his junk • Females have rounded abdomens Female Male

  11. Sexing Female Male

  12. Our Treatments • Control group: • 5 white-eyed males, 5 white females • Experimental group: • 5 white-eyed males, 5 white females, 1 red male (“mutant”)

  13. Started with this… Control group: 5 white-eyed males, 5 white females Experimental group: 5 white-eyed males, 5 white females, 1 red male

  14. After 1 Generation, you will sort the flies On a white piece of paper…. Sort into categories and count

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