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PCR

PCR. By: Cody Alveraz Ted Dobbert Morgan Pettit . What is PCR?. PCR is a polymerase chain reaction that copies pieces of DNA across multiple orders of magnitude, creating thousands to millions of copies of DNA sequences. It was developed in 1983 by Kary Mullis.

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PCR

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  1. PCR By: Cody Alveraz Ted Dobbert Morgan Pettit

  2. What is PCR? • PCR is a polymerase chain reaction that copies pieces of DNA across multiple orders of magnitude, creating thousands to millions of copies of DNA sequences. • It was developed in 1983 by Kary Mullis.

  3. Procedures of PCR • Denaturation: At 201.2 F degrees the double stranded DNA melts and opens into 2 pieces of single-stranded DNA. • Annealing: At medium temperatures, at around 54 degrees Celsius, the primers pair up with the single-stranded “template”. On the small length of double-stranded DNA, the polymerase attaches and starts copying the template.

  4. Procedures of PCR (cont.) • Extension: AT 72 degrees Celsius, where the polymerase works best, and DNA building blocks complementary to the template are coupled to the primer, making a double-stranded DNA molecule. • With one cycle a segment of double-stranded DNA template is amplified into two separate pieces of double stranded DNA.

  5. What is good about PCR? • PCR is good technique because its not expensive, rapid and a good way to get millions of copies of DNA.

  6. PCR Process Diagram

  7. PCR Machine

  8. Links: • http://www.medicinenet.com/pcr_polymerase_chain_reaction/article.htm • http://www.youtube.com/watch?v=eEcy9k_KsDI

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