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PCR PowerPoint Presentation

PCR

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PCR

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  1. PCR

  2. PCR AMPLIFICATION

  3. PCR APPLICATIONS • MOLECULAR BIOLOGY • ENVIRONMENTAL SCIENCE • FORENSIC/MEDICAL SCIENCE • BIOTECHNOLOGY • GENETICS • GENE CLONING • MICROBIOLOGY

  4. DNA STRUCTURE • SUGAR-PHOSPHATE BACKBONE • A=ADENINE • T=THYMINE • C=CYTOSINE • G=GUANINE

  5. PCR REQUIREMENTS • DNA • PRIMERS • dATP, dTTP, dCTP, dGTP • POLYMERASES

  6. RAPD PCR • RANDOM • AMPLIFIED • POLYMORPHIC • DNA

  7. RAPD APPLICATIONS • DNA FINGERPRINTING • ANALYSIS OF ANCIENT DNA FROM FOSSILS • MAPPING THE HUMAN GENOME • FINGERPRINTING UNKNOWN MICROORGANISMS

  8. RAPD PRIMERS • 10 NUCLEOTIDES LONG • ARBITRARY SEQUENCE • BINDS AT MULTIPLE SEQUENCES ALONG DNA • NON-SPECIFIC BINDING • ACCURACY REQUIRES AT LEAST 10 PRIMERS