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Brainstorming …

Brainstorming …. Concerns. Environmental or medicinal Applicable/relevant Novel???? Elegant???? Recombinant/biosynthetic work. Possibilities. Plastic/cellulose conversion to biodiesel via microbes and/or their products Enzymatic degradation of biofilm via toothpaste reagents

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Brainstorming …

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  1. Brainstorming…

  2. Concerns Environmental or medicinal Applicable/relevant Novel???? Elegant???? Recombinant/biosynthetic work

  3. Possibilities Plastic/cellulose conversion to biodiesel via microbes and/or their products Enzymatic degradation of biofilm via toothpaste reagents Quick indication of successful transformation (such as floating bacteria)

  4. Others… Targeting termites via microbiot-icides Temp-sensitive chromo/fluorescent microbe (thermometer) Chromosome counter Fluorophores and quenchers…

  5. Intriguing Melanoma/SCC/BCC detection… -currently, visual and biopsy Hypothetical -visual, chromotest, biopsy

  6. But how…? Initial thoughts: -differential -live vs. dead (trypan blue stain) -mitotic index (DNA Hoechst stain) -keratin differentiation (protein Ponceau S stain) How to build in an assay???

  7. HPV Detection • Irregular growth & acetic acid test • Overexpress putatitive HPV receptor (HSPG) on S. epidermidis • Link it intracellular amplification regimen • CRE recombinase is expressed • Excises floxed E. chromi color • Color is lost as floxed color is removed from promoter

  8. Cell Aging • Telomere length = division surrogate • Circadian rhythm events??? • Protein profiles???

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