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Analysis of Gene Expression in Various Cell Lines via RT-PCR and Methylation Assessment

This study explores the expression levels of different genes including A. Rael, Dante, and Ramos across various cell lines such as DG75, Mutu, and Lepre1. Techniques utilized include MSP and RT-PCR to evaluate methylation status and gene expression percentages. The results reveal significant variations in methylation across cell lines, categorized into ranges of 0-5%, 6-10%, 11-20%, 21-50%, and 51-100%, providing insights into the epigenetic regulation of these genes.

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Analysis of Gene Expression in Various Cell Lines via RT-PCR and Methylation Assessment

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  1. A Rael Dante Ramos DG75 Mutu LCL LCL LCL LCL Lepre1 GAPDH LK6 JUM2 DoHH1 DoHH2 DHL-4 DHL-7 CRL SUD-4 B CU CM U M C D CpG 1 2 3 4 5 6 7 8 Mean % MSP RT-PCR 1.7 U + PF + 95.8 0 + U BM + Akata 0 + U C2 + BL16 + 0 U Ramos + U AK31 0 + 0 U DG75 + U 0 BL2 0 + BL41 U + U 0 DoHH2 0 + SUD-4 U 0-5% 6-10% 11-20% 21-50% 51-100%

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