'Cell lines' diaporamas de présentation

Review from last time

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RIGOR AND REPRODUCIBILITY: BACK TO BASICS

RIGOR AND REPRODUCIBILITY: BACK TO BASICS. Patricia Valdez, Ph.D. NIH Extramural Research Integrity Officer National Institutes of Health. Overview. Why the concern about reproducibility? The NIH response Updates to grant applications Training and Resources. Overview.

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Cell Types, Culture Characteristics, Tissue Culture

Cell Types, Culture Characteristics, Tissue Culture. Primary Cultures. Derived directly from excised, normal animal tissue and cultures either as explant culture or following dissociation into a single cell suspension by enzyme digestion.

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EST EXPRESSION PROFILE ANALYSIS ??? ????

EST EXPRESSION PROFILE ANALYSIS ??? ????. ????????????? ????????????? . EST. E xpressed S equence T ags. EST. E xpressed S equence T ags are partial , single-pass sequences from either end of a cDNA clone. .

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Agenda

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Apoptotic activity of Vitamin E Phosphate: A novel strategy in the cancer chemotherapy

Apoptotic activity of Vitamin E Phosphate: A novel strategy in the cancer chemotherapy. Dr. Bashir M. Rezk. Biological activity of VE. Vitamin E derivatives. VE is often supplemented as a chemically stable ester derivative such as Vitamin E Acetate (VEA) Vitamin E Succinate (VES)

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Pharmacogenetics in the treatment of breast and ovarian cancer patients

Pharmacogenetics in the treatment of breast and ovarian cancer patients. Peter A. Fasching. UCLA. David Geffen School of Medicine Div. Hem/Onc. Concepts of science. Therapy A. Therapy B. Therapy A must be better. Pitfalls with this approach. Will this patient have a recurrence?.

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Characterisation of the KIR genes in HLA homozygous cell lines Christian Garcia

Anthony Nolan Research Institute Royal Free Hospital School of Medicine University College London. Characterisation of the KIR genes in HLA homozygous cell lines Christian Garcia. Introduction.

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Evaluation of Viral Clearance Studies

Evaluation of Viral Clearance Studies. Mahmood Farshid, Ph.D. Div. Of Hematology OBRR/ CBER/FDA. Biologics . Monoclonal antibodies and recombinant products produced in cell culture Blood and blood products and other human derived products Animal derived products.

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MLAB 1415-Hematology Keri Brophy-Martinez

MLAB 1415-Hematology Keri Brophy-Martinez. Chapter 13: Hypoproliferative Anemias. Overview. Disorders Acquired Congenital Anemia Normocytic or Macrocytic Normochromic Bone marrow hypocellularity Defect Depletion, damage or inhibition of stem and/or progenitor cells. APLASTIC ANEMIA.

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Biology of cell in-vitro

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Baculovirus-Mediated Expression in Mammalian Cells.

Baculovirus-Mediated Expression in Mammalian Cells. 5 / 27 / 2003 Jong-Chan, AHN. Interdisciplinary Graduate Program in Genetic Engineering (IGPGE). Enhancement and Prolongation of Baculovirus-Mediated Expression in Mammalian Cells: Focuses on Strategic Infection and Feeding.

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PAPILLOMA VIRUSES

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Identification of ER ? gene polymorphism in adolescent idiopathic scoliosis (AIS)

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Figure S1

Vasilatos et al . Relative LSD1 mRNA (%) (HDAC siRNA vs. Scramble) . MDA-MB-231 cells. Figure S1. Vasilatos et al . NR4A1. mRNA level (Fold) Relative to MDA-MB-231 . PCDH1. mRNA level (Fold) Relative to MDA-MB-231 . RGS16. mRNA level (Fold) Relative to MDA-MB-231 . BIK.

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Coming Soon... Mandatory Biosafety Training

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Mean fluorescence intensity

Negative control. E-cadherin. KM12C shRNA SCRAMBLE. KM12C shRNA CDH17. 10 1. 10 1. 10 1. 10 1. 10 2. 10 2. 10 2. 10 2. 0. 0. 0. 0. 10 3. 10 3. 10 3. 10 3. 10 4. 10 4. 10 4. 10 4. Cell number. Cell number. Mean fluorescence intensity. Mean fluorescence intensity.

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mKap binds specifically to a range of MM ? cell lines

A. Only one surviving mouse. p<0.0001. p<0.005. n =6 for all groups. B. A. B. mKap. cKap. Association Rate Constant K a (M -1 s -1 ). 3.64x10 4. 3.40x10 4. Dissociation Rate Constant K d (s -1 ). 7.44x10 -4. 7.58x10 -4. K A (M -1 ). 4.87x10 7. 4.48x10 7. Equilibrium

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Dual mTOR/HDAC inhibition: preclinical development of a novel breast cancer therapy

Dual mTOR/HDAC inhibition: preclinical development of a novel breast cancer therapy Mariya Yevtushenko, Kathleen A. Wilson- Edell , and Christopher C. Benz Buck Institute for Research on Aging, Novato, CA. Background. 2 . MLN0128 and TSA synergistically decrease breast cancer cell viability.

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Myo - blasts RMZ (Bio- RH3 -RC2 Heart) RMZ-RC2

Supplementary figure 1

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