1 / 9

Gene Expression in Early Stages of Micorrhizal Interactions

Gene Expression in Early Stages of Micorrhizal Interactions. By Sahel Arora. a mutualistic symbiosis between plant and fungus 2 major types Endomycorrhizae-- fungal component are Glomales Endomycorrhizae -- hyphae penetrates the outer cells of plant root. What is Mycorrhizae - review.

cece
Télécharger la présentation

Gene Expression in Early Stages of Micorrhizal Interactions

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Gene Expression in Early Stages of Micorrhizal Interactions By Sahel Arora

  2. a mutualistic symbiosis between plant and fungus 2 major types Endomycorrhizae-- fungal component are Glomales Endomycorrhizae --hyphae penetrates the outer cells of plant root What is Mycorrhizae - review

  3. Review -- Ectomycorrhizae • associated plant species include majority of vascular plants • Essential for health and survival of conifers and hardwood species • the function depends on the ability of the fungal symbionts to take up nutrients

  4. Ectomycorrhizal Organ • Formation has 4 stages • Time of the process varies • Laccaria bicolor – Pinus resinosa interaction takes eight weeks • Pisolithus tinctorius – Eucalyptus globus takes about two weeks

  5. Fungal gene expression in early symbiotic interactions between Laccaria bicolor and red pine • Authors – G.K. Podila, J. Zheng, S. Balasubramaniam, S. Sundaram, S. Hiremath, J.H. Brand, and M.K. Hymes • Aim – to obtain a broad overview on various genes involved in the pre-infection stages of ectomycorrhizal formation, in the fungal partner • Uses L. bicolor for the fungal component and Pinus resinosa for the plant • it is known that signaling genes are involved in the early stage, the types of genes are unknown

  6. Materials and Methods • L. Bicolor was obtained, grown and maintained on MMN medium • In vitro system was used to isolate and analyze genes • cDNA library was constructed • cDNA – complimentary DNA; synthesized by using reverse transcriptase and ohage vector • cDNAs represent mRNAs during: a) its interaction with red pines for 6 – 72 hrs; b) free-living conditions

  7. Materials and Methods • suppression subtractive hybridization was used to generate cDNAs, enriched in IS sequences • These sequences were used as probes to screen the actions of cDNA • The resulting clones were converted to plasmid clones and purified • The clones went through the membrane array analyses and northern analyses for validation

  8. RESULTS AND DISCUSSION • The analyses indicated a relative increase in the expression of each gene in the interaction over the free-living state • Also, showed an increase in levels of clones tested, however there was a variation • The data identified several regulatory genes and insights into gene activities involved

More Related