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2-amino-3-carboxymuconate-6-semialdehyde

2-amino-3-carboxymuconate-6-semialdehyde. 3-hydroxyanthranilate dioxygenase. If you need nictotinamide it ends here. Assume loss of NADH. + NH 3. Trp = 12 ATP - 2 NADPH - (NADH?) + NADH +  -ketoadipate + NH 3.

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2-amino-3-carboxymuconate-6-semialdehyde

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  1. 2-amino-3-carboxymuconate-6-semialdehyde 3-hydroxyanthranilate dioxygenase

  2. If you need nictotinamide it ends here

  3. Assume loss of NADH

  4. + NH3 Trp = 12 ATP - 2 NADPH - (NADH?) + NADH + -ketoadipate + NH3

  5. -ketoadipate dehydrogenase Same mechanism as pyruvate dehydrogenase and -ketoglutarate dehydrogenase.

  6. -ketoadipate = NADH + glutaryl-CoA

  7. -ketoadipate = NADH + FADH2 + glutaconyl-CoA

  8. -ketoadipate = 2 NADH + FADH2 + Acetoacetyl-CoA

  9. -ketoadipate = 2 NADH + FADH2 + 2 Acetyl-CoA

  10. So…Trp = 12 ATP - 2 NADPH + NH3+ -ketoadipate If: -ketoadipate = 2 NADH + FADH2 + 2 Acetyl-CoA If: Acetyl-CoA = 3 NADH + FADH2 + GTP Then: -ketoadipate = 8 NADH + 3 FADH2 + 2 GTP Then: Trp = 12 ATP + 8 NADH + 3 FADH2 + 2 GTP - 2 NADPH + NH3 Remember malate dehydrogenase + malic enzyme converts NADH + NADP+ ---> NAD+ + NADPH So….-2 NADPH = -2 NADH Then: Trp = 12 ATP + 6 NADH + 3 FADH2 + 2 GTP + NH3 If GTP = ATP, NADH = 3 ATP FADH2 = 2 ATP and NH3 = -2 ATP Then: Trp = 36 ATP + N10-formyl-THF

  11. Homogenistate dioxygenase

  12. Makes fumarate + acetoacetate at the expense of NADPH and ascorbate

  13. So…Phe = fumarate + acetoacetate - NADPH - Asc + NH3 and Tyr = fumarate + acetoacetate - Asc + NH3 If: fumarate = malate Then: Phe = malate + acetoacetate - NADPH - Asc + NH3 and Tyr = malate + acetoacetate - Asc + NH3 If: malate = pyruvate - NADH + NADPH Then: Phe = pyruvate + acetoacetate - NADH - Asc + NH3 and Tyr = pyruvate + acetoacetate - Asc + NH3 - NADH + NADPH If: acetoacetate = 2 Acetyl-CoA - ATP Then: Phe = pyruvate + 2 Acetyl-CoA - NADH - Asc + NH3 -ATP and Tyr = pyruvate + 2 Acetyl-CoA - Asc + NH3 - NADH + NADPH - ATP If: pyruvate = 4 NADH + FADH2 + GTP If: Acetyl-CoA = 3 NADH + FADH2 + GTP Then: Phe = 9 NADH + 3 FADH2 + 2 GTP - Asc + NH3 and Tyr = NH3 + 2 GTP + 3 FADH2 + 9 NADH + NADPH - Asc

  14. Then: Phe = 9 NADH + 3 FADH2 + 2 GTP - Asc + NH3 and Tyr = NH3 + 2 GTP + 3 FADH2 + 9 NADH - NADPH - Asc If: Asc = - NADPH Then: Phe = 9 NADH + 3 FADH2 + 2 GTP - NADPH + NH3 and Tyr = NH3 + 2 GTP + 3 FADH2 + 9 NADH - 2 NADPH If: NADPH = NADH Then: Phe = 8 NADH + 3 FADH2 + 2 GTP + NH3 and Tyr = NH3 + 2 GTP + 3 FADH2 + 7 NADH If GTP = ATP, NADH = 3 ATP FADH2 = 2 ATP and NH3 = -2 ATP Then: Phe = 30 ATP Tyr = 27 ATP

  15. Histidine degradation Histidine Ammonia lyase Urocanase Urocanase Urocanase Urocanase Urocanase

  16. Imidazolone propionase Glutamate formiminotransferase

  17. So….Histidine = glutamate + N5-formimino-THF If: Glu = -ketoglutarate + NADPH + NH3 Then: His = -ketoglutarate + NADPH + NH3 If: -ketoglutarate = 4 NADH + 2 GTP + 2 FADH2 + NADPH Then: His = 4 NADH +2 GTP + 2 FADH2 + NADPH + NH3 If: NADH = 3 ATP GTP = ATP FADH2 = 2 ATP NH3 = -2 ATP Then: His = 16 ATP + NADPH + N5-formimino-THF

  18. Amino acids as precursors for biological molecules

  19. Nucleic Acid Biosynthesis

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