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Analysis of MDH and SOD Activities in Jurkat Cells and Rat Heart Fractions

This research investigates the activity levels of malate dehydrogenase (MDH) and superoxide dismutase (SOD) in Jurkat cells and fractions of rat heart tissues. The study evaluates the performance of various cells and fractions, revealing quantitative metrics such as MDH activity (%), SOD activity (%) across different samples, including comparative assessments between Jurkat cells and heart tissues. Results presented in Figures S1-S4, and detailed in Table S1, contribute valuable insights into cellular enzymatic functions and potential implications in metabolic studies.

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Analysis of MDH and SOD Activities in Jurkat Cells and Rat Heart Fractions

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  1. kBp 23.1 9.4 6.5 4.3 2.3 2.0 kBp 10 6 4 3 2 Figure S1 P1-Fraction 3 P2-Fraction 1 M 1 2 M 1 2

  2. Figure S2 A JURKAT P1/S1

  3. Figure S2 B JURKAT P2

  4. Figure S2 C Rat Heart P1/S1

  5. Figure S2 D Rat Heart P2

  6. Figure S3

  7. Figure S4

  8. Rat Heart 100 0.17 ± 0.075 0.22 ± 0.05 100 69 ± 5 63 ± 2.2 100 0.43 ± 0.125 0.29 ± 0.126 Jurkat Cells 100 61 ± 3.8 60 ± 2.5 MDH activity in % SOD activity in % P1 S P1 P2 Fraction S P2 Table S1

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