1. Go to the STRING web sitehttp://string.embl.de Lookup examples Judge the correctness of the predictions Use the evidence viewers to understand the basis for the predictions Feel free to also lookup proteins that you are interested in or to use the ”random” link Examples for “Protein mode” MURC_TREPA ANP1_YEAST Examples for “COG mode” COG0055 COG1117 Examples for comparing the two different modes trpB P91427 Yeast CDC28 Human CDC2 and CDK2 STRING examples

2. Go to the following services GlobPlothttp://globplot.embl.de DisEMBLhttp://dis.embl.de ELMhttp://elm.eu.org ProtFunhttp://www.cbs.dtu.dk/services/ProtFun/ Submit the following examples and interpret the results PRIO_HUMAN P53_HUMAN Go to the following services TargetPhttp://www.cbs.dtu.dk/services/TargetP/ PSORThttp://psort.nibb.ac.jp/ ExPASy ProtParamhttp://www.expasy.org/tools/protparam.html Try to predict the subcellular localization of these calpains CANA_HUMAN CAN1_HUMAN (CAN2_HUMAN) Examples for ab initio prediction methods