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This study investigates the gene expression patterns in Drosophila using Gal4 drivers and specific genetic variants, including γGFP and VCHth. We explore the roles of key proteins such as Hth, Exd, and VNE in developmental processes and localization within tissues. The research utilizes various constructs, including PBCA and HD variants, to visualize expression through fluorescence markers. Results provide insights into the regulatory networks involving Hth and associated factors. Additionally, supplementary files are included for comprehensive data analysis.
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A C B enGal4 > enGal4 > γGFP Hth Exd HthVN VCE HD PBCA HM HD VNE HthVN HthVN VCHth VCE VCHth VNE γGFP VCHth Additional File 3