1 / 19

Understanding DNA Repair and Mutagenesis in Microbial Genetics

Lecture 14 of Microbial Genetics (Micr340) explores the critical concepts of DNA repair and mutagenesis. Key topics include the definition of lesions and mutagens, evidence for DNA repair mechanisms, and specific repair pathways. The lecture delves into the repair of deaminated bases, damage caused by reactive oxygen species, alkylation of DNA, and the crucial process of pyrimidine dimer repair. Additionally, it covers various repair mechanisms such as methyl-directed mismatch repair, nucleotide excision repair, and the SOS response in E. coli.

cheri
Télécharger la présentation

Understanding DNA Repair and Mutagenesis in Microbial Genetics

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Microbial Genetics (Micr340) Lecture 14 DNA Repair and Mutagenesis

  2. Definitions • Lesion: chemical damage in DNA • Mutagens: chemical compounds that cause lesions in DNA which can kill cells and can also increase frequency of mutations in DNA

  3. Evidence for DNA repair

  4. Specific Repair Pathways • Repair of deaminated bases • Damage due to reactive oxygen • Alkylation of DNA and repair • Pyrimidine dimer repair

  5. Base Deamination by nitrous acid

  6. Spontaneous Base Deamination

  7. Repair of altered bases by DNA glycosylases

  8. Damage due to reactive oxygen

  9. Mechanism of avoiding GO

  10. AlkylationOf guanine

  11. Repair of alkylated DNA • Specific N-glycosylases • Methyltransferases

  12. PyrimidineDimer Formation

  13. Photoreactivation

  14. General Repair Mechanisms • Methyl-directed mismatch repair system • Nucleotide excision repair • SOS inducible repair

  15. A model for methylDirected mismatch repair

  16. A model for nucleotide excision repair

  17. SOS response

  18. SOS response

  19. Regulation of SOS mutagenesis in E. coli

More Related