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Advanced Techniques in Linear Dichroism Spectrometry for Molecular Orientation Studies

Explore polarization modulation with photoelastic modulator (PEM) for Circular Dichroism (CD) analysis. Utilize Linear Dichroism - Reflection (IRRAS) with UV or IR spectrometer and variable angle of incidence. Study peptides and proteins in membranes using Langmuir-trough setup. Discover the complexities of polarization measurements in crystal samples for FTIR spectroscopy.

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Advanced Techniques in Linear Dichroism Spectrometry for Molecular Orientation Studies

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  1. Polarization Modulation with PEM for CD (courtesy Hinds Instr.)

  2. Linear Dichroism Schematic(courtesy Hinds Instr.) Spectrometer UV or IR Linear polarizer PEM

  3. Variable angle of incidence Linear Dichroism - Reflection (IRRAS) (courtesy Hinds Instruments) Surface can be metal (high reflection, often grazing incidence) or dielectric, or even air-water interface For film on liquid use Langmuir-trough for sample--studies of peptides and proteins in membranes possible, orientation from polarization

  4. ATR Polarization Measurements IR beam multiply reflectsinside crystal -- penetrates surface keeps polarizations: Eperp in surface, Epara partially out In from FTIR E Crystal- Typ: Ge, ZnSe High index for refl. out Sample coated on crystal

  5. Schematic – External reflectance FTIR & L-B trough Dluhy, App.Spec.Rev.2000

  6. z E|| z B|| x B| E y x n = c/l l Light—Electro-Magnetic (E-M) radiation Tool for probing quantum mechanical states k|| y

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