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John 15:4

John 15:4 4 Abide in me, and I in you. As the branch cannot bear fruit of itself, except it abide in the vine; no more can ye, except ye abide in me. From Sequence to Chromosome: The Tip of the X Chromosome of D. Melanogaster. Timothy G. Standish, Ph. D. The Drosophila Genome.

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John 15:4

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  1. John 15:4 4 Abide in me, and I in you. As the branch cannot bear fruit of itself, except it abide in the vine; no more can ye, except ye abide in me

  2. From Sequence to Chromosome:The Tip of the X Chromosome of D. Melanogaster Timothy G. Standish, Ph. D.

  3. The Drosophila Genome • 180 million base pairs • 120 Mbp has been sequenced • The sequenced portion is euchromatin thought to be richer in gene sequences than the remaining heterochromatin • Drosophila has a haploid number of 4: 3 autosomes plus 1 sex chromosome • Polytene chromosomes can be easily observed in the salivary glands of larvae

  4. Telomere Distal Stained polytene chromosomes exhibit distinctive banding patterns Arm Proximal Centromere Proximal Arm Distal Telomere Chromosome Morphology

  5. Telomere Arm Centromere Arm Telomere Chromosome Morphology During development chromosome puffs appear which are thought to be correlated with transcriptional activity

  6. Naming Chromosome Parts • Chromosomes are numbered 1 - 3 X and Y • Each chromosome is divided into left (L) and Right (R) arms • Arms are divided into numbered divisions going left to right starting with the X chromosome distal left arm and ending with division 100 on the distal right arm of chromosome 3. • Divisions are divided into lettered subdivisions going left to right

  7. Drosophila X Chromosome 2b • In division 2b of the X chromosome, a strange bulge appears in images of polytene chromosomes • Insitu hybridization using cosmid clones mapped to that region show hybridization on the outside of this structure, but not in the middle Figure from http://www.helsinki.fi/~saura/EM/xdiv2.html

  8. Cosmids • A cosmid is a plasmid with the l page cos site included in it • The cos site signals for packaging into l phage, thus the plasmid, including a large insert can be packaged into phage particles without having to waste space on l genes • A cosmid combined with a cloned insert cannot exceed the 53 kb limit on how much can be packaged into the l phage head

  9. 3.5 repeats of satellite like element 350 bp An Inverted Repeat May Account For Strange Morphology At X2B • These repeated elements resemble the 1.688 satellite found in units of 1 - 4 on the X chromosome • Results indicate that inverted repeats can influence chromosome architecture even when separated by significant distances

  10. The End

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