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Evolutionary tree for fish species

Sample isolation for SDS-PAGE. SDS and heat disrupt 2

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Evolutionary tree for fish species

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    1. Evolutionary tree for fish species

    2. Sample isolation for SDS-PAGE

    3. SDS-PAGE gel

    4. WEEK 1: Isolating the Protein

    5. Identifying Similarities Between Species Using Protein Gel Electrophoresis

    6. Week 2: Analyzing our gels

    7. Equations for size MW of protein = # of aa x 110D/aa Actin: 42000D = # of aa x 110D/aa ~382 aa MW of gene (kD) = ((# of aa x 3 codons/aa) x 660D/base pair)/1000 ~382 x 3 x 660/1000 = 756.3kD (size of actin gene)

    8. Equations for commonality Percent commonality = (# proteins in common between 2 species)/(total # of proteins for both species) x 100% Percent commonality = (12 similar bands)/(37 bands total) x 100% ~32%

    9. Kaleidoscope standard

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