1 / 55

Biometrical genetics

Biometrical genetics. Manuel Ferreira Shaun Purcell Pak Sham. Boulder Introductory Course 2006. Outline. 1. Aim of this talk. 2. Genetic concepts. 3. Very basic statistical concepts. 4. Biometrical model. 1. Aim of this talk.

daphne
Télécharger la présentation

Biometrical genetics

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Biometrical genetics Manuel Ferreira Shaun Purcell Pak Sham Boulder Introductory Course 2006

  2. Outline 1. Aim of this talk 2. Genetic concepts 3. Very basic statistical concepts 4. Biometrical model

  3. 1. Aim of this talk

  4. Revisit common genetic parameters - such as allele frequencies, genetic effects, dominance, variance components, etc Use these parameters to construct a biometrical genetic model • Model that expresses the: • Mean • Variance • Covariance between individuals • for a quantitative phenotype as a function of genetic parameters.

  5. [0.25/1] [0.5/1] 1 1 1 1 1 1 E D A A D E e d a a d e PT1 PT2

  6. 2. Genetic concepts

  7. G G G G G G Population level G G G Allele and genotype frequencies G G G G G G G G G G G Transmission level G G Mendelian segregation Genetic relatedness G G Phenotype level Biometrical model P P Additive and dominance components

  8. Population level 1. Allele frequencies • A single locus, with two alleles • - Biallelic / diallelic • - Single nucleotide polymorphism, SNP A a Alleles A and a - Frequency of A is p - Frequency of a is q = 1 – p A a Every individual inherits two alleles - A genotype is the combination of the two alleles - e.g. AA, aa (the homozygotes) or Aa (the heterozygote)

  9. Population level 2. Genotype frequencies (Random mating) Allele 1 A (p) a (q) A (p) AA (p2) Aa (pq) Allele 2 a (q) aa (q2) aA (qp) Hardy-Weinberg Equilibrium frequencies P (AA) = p2 P (Aa) = 2pq p2+ 2pq + q2 = 1 P (aa) = q2

  10. Transmission level 1. Mendel’s experiments AA aa Pure Lines F1 Aa Aa Intercross AA Aa Aa aa 3:1 Segregation Ratio

  11. Transmission level F1 Pure line Aa aa Back cross Aa aa 1:1 Segregation ratio

  12. Transmission level AA aa Pure Lines F1 Aa Aa Intercross Aa Aa aa AA 3:1 Segregation Ratio

  13. Transmission level F1 Pure line Aa aa Back cross Aa aa 1:1 Segregation ratio

  14. Transmission level 1. Mendel’s law of segregation Mother (A3A4) Segregation, Meiosis Gametes A3(½) A4(½) A1(½) A1A3(¼) A1A4(¼) Father (A1A2) A2(½) A2A4(¼) A2A3(¼)

  15. Phenotype level 1. Classical Mendelian traits Dominant trait (D - presence, R - absence) - AA, Aa D - aa R Recessive trait (D - absence, R - presence) - AA, Aa D - aa R Codominant trait (X, Y, Z) - AA X - Aa Y - aa Z

  16. Phenotype level 2. Dominant Mendelian inheritance Mother (Dd) D(½) d(½) D (½) DD(¼) Dd(¼) Father (Dd) d (½) dd(¼) dD(¼)

  17. Phenotype level 3. Dominant Mendelian inheritance with incomplete penetrance and phenocopies Mother (Dd) D(½) d(½) DD(¼) Dd(¼) D (½) Father (Dd) Incomplete penetrance dD(¼) dd(¼) d (½) Phenocopies

  18. Phenotype level 4. Recessive Mendelian inheritance Mother (Dd) D(½) d(½) D (½) DD(¼) Dd(¼) Father (Dd) d (½) dd(¼) dD(¼)

  19. AA Aa aa Phenotype level 5. Quantitative traits

  20. Phenotype level P(X) Aa Biometric Model aa AA X aa Aa AA m +a d – a Genotypic effect m – a m + d m + a Genotypic means

  21. 3. Very basic statistical concepts

  22. Mean, variance, covariance 1. Mean (X)

  23. Mean, variance, covariance 2. Variance (X)

  24. Mean, variance, covariance 3. Covariance (X,Y)

  25. 4. Biometrical model

  26. Biometrical model for single biallelic QTL Biallelic locus - Genotypes: AA, Aa, aa - Genotype frequencies: p2, 2pq, q2 Allele 1 A (p) a (q) Genotype frequencies (Random mating) A (p) AA (p2) Aa (pq) Allele 2 a (q) aa (q2) aA (qp) Hardy-Weinberg Equilibrium frequencies P (AA) = p2 P (Aa) = 2pq p2+ 2pq + q2 = 1 P (aa) = q2

  27. Biometrical model for single biallelic QTL Biallelic locus - Genotypes: AA, Aa, aa - Genotype frequencies: p2, 2pq, q2 Alleles at this locus are transmitted from P-O according to Mendel’s law of segregation Genotypes for this locus influence the expression of a quantitative trait X (i.e. locus is a QTL) Biometrical genetic modelthat estimates the contribution of this QTL towards the (1) Mean, (2) Variance and (3) Covariancebetween individuals for this quantitative trait X

  28. Phenotype level P(X) Aa Biometric Model aa AA X aa Aa AA m +a d – a Genotypic effect m – a m + d m + a Genotypic means

  29. Biometrical model for single biallelic QTL 1. Contribution of the QTL to the Mean (X) Genotypes Aa aa AA a d -a Effect, x p2 2pq q2 Frequencies, f(x) Mean (X) = a(p2) + d(2pq) – a(q2) = a(p-q) + 2pqd

  30. Biometrical model for single biallelic QTL 2. Contribution of the QTL to the Variance (X) Genotypes Aa aa AA a d -a Effect, x p2 2pq q2 Frequencies, f(x) Var (X) = (a-m)2p2 + (d-m)22pq + (-a-m)2q2 = VQTL Broad-sense heritability of X at this locus = VQTL/ V Total Broad-sense total heritability of X = ΣVQTL/ V Total

  31. Biometrical model for single biallelic QTL Var (X) = (a-m)2p2 + (d-m)22pq + (-a-m)2q2 =2pq[a+(q-p)d]2 + (2pqd)2 = VAQTL+ VDQTL Additive effects: the main effects of individual alleles Dominance effects: represent the interaction between alleles d = 0 aa Aa AA m +a d – a

  32. Biometrical model for single biallelic QTL Var (X) = (a-m)2p2 + (d-m)22pq + (-a-m)2q2 =2pq[a+(q-p)d]2 + (2pqd)2 = VAQTL+ VDQTL Additive effects: the main effects of individual alleles Dominance effects: represent the interaction between alleles d > 0 aa Aa AA m +a d – a

  33. Biometrical model for single biallelic QTL Var (X) = (a-m)2p2 + (d-m)22pq + (-a-m)2q2 =2pq[a+(q-p)d]2 + (2pqd)2 = VAQTL+ VDQTL Additive effects: the main effects of individual alleles Dominance effects: represent the interaction between alleles d < 0 aa Aa AA m +a d – a

  34. a d m -a Biometrical model for single biallelic QTL aa Aa AA Var (X) = Regression Variance + Residual Variance = Additive Variance + Dominance Variance

  35. Practical H:\manuel\Biometric\sgene.exe

  36. Practical Aim Visualize graphically how allele frequencies, genetic effects, dominance, etc, influence trait mean and variance Ex1 a=0, d=0, p=0.4, Residual Variance = 0.04, Scale = 2. Vary a from 0 to 1. Ex2 a=1, d=0, p=0.4, Residual Variance = 0.04, Scale = 2. Vary d from -1 to 1. Ex3 a=1, d=0, p=0.4, Residual Variance = 0.04, Scale = 2. Vary p from 0 to 1. Look at scatter-plot, histogram and variance components.

  37. Some conclusions • Additive genetic variance depends on allele frequency p & additive genetic value a as well as dominance deviation d • Additive genetic variance typically greater than dominance variance

  38. Biometrical model for single biallelic QTL Var (X) =2pq[a+(q-p)d]2 + (2pqd)2 Demonstrate VAQTL+ VDQTL 2A. Average allelic effect 2B. Additive genetic variance

  39. Biometrical model for single biallelic QTL 1. Contribution of the QTL to the Mean (X) 2. Contribution of the QTL to the Variance (X) 2A. Average allelic effect (α) 2B. Additive genetic variance 3. Contribution of the QTL to the Covariance (X,Y)

  40. Biometrical model for single biallelic QTL 3. Contribution of the QTL to the Cov (X,Y) (a-m) Aa aa (-a-m) (d-m) AA (a-m)2 (a-m) AA (d-m) (a-m) (d-m) (d-m)2 Aa aa (-a-m)2 (-a-m) (-a-m) (d-m) (-a-m) (a-m)

  41. Biometrical model for single biallelic QTL 3A. Contribution of the QTL to the Cov (X,Y) – MZ twins (a-m) Aa aa (-a-m) (d-m) AA p2 (a-m)2 (a-m) AA (d-m) (a-m) (d-m) (d-m)2 Aa 0 2pq aa (-a-m)2 (-a-m) (-a-m) (d-m) (-a-m) q2 0 (a-m) 0 Covar (Xi,Xj) = (a-m)2p2 + (d-m)22pq + (-a-m)2q2 = VAQTL+ VDQTL =2pq[a+(q-p)d]2 + (2pqd)2

  42. Biometrical model for single biallelic QTL 3B. Contribution of the QTL to the Cov (X,Y) – Parent-Offspring (a-m) Aa aa (-a-m) (d-m) AA p3 (a-m)2 (a-m) AA (d-m) (a-m) (d-m) (d-m)2 Aa p2q pq aa (-a-m)2 (-a-m) (-a-m) (d-m) (-a-m) q3 0 (a-m) pq2

  43. e.g. given an AAfather, an AAoffspring can come from either AAx AAor AAx Aaparental mating types AAx AA will occur p2× p2 = p4 and have AA offspring Prob()=1 AAx Aa will occur p2× 2pq = 2p3q and have AA offspring Prob()=0.5 and have Aa offspring Prob()=0.5 Therefore, P(AA father & AAoffspring) = p4 + p3q = p3(p+q) = p3

  44. Biometrical model for single biallelic QTL 3B. Contribution of the QTL to the Cov (X,Y) – Parent-Offspring (a-m) Aa aa (-a-m) (d-m) AA p3 (a-m)2 (a-m) AA (d-m) (a-m) (d-m) (d-m)2 Aa p2q pq aa (-a-m)2 (-a-m) (-a-m) (d-m) (-a-m) q3 0 (a-m) pq2 Cov (Xi,Xj) = (a-m)2p3 + … + (-a-m)2q3 = ½VAQTL =pq[a+(q-p)d]2

  45. Biometrical model for single biallelic QTL 3C. Contribution of the QTL to the Cov (X,Y) – Unrelated individuals (a-m) Aa aa (-a-m) (d-m) AA p4 (a-m)2 (a-m) AA (d-m) (a-m) (d-m) (d-m)2 Aa 2p3q 4p2q2 aa (-a-m)2 (-a-m) (-a-m) (d-m) (-a-m) q4 p2q2 (a-m) 2pq3 Cov (Xi,Xj) = (a-m)2p4 + … + (-a-m)2q4 = 0

  46. Biometrical model for single biallelic QTL 3D. Contribution of the QTL to the Cov (X,Y) – DZ twins and full sibs ¼ genome ¼ genome ¼ genome ¼ genome # identical alleles inherited from parents 2 1 (father) 0 1 (mother) ¼ (2 alleles) + ½ (1 allele) + ¼ (0 alleles) Unrelateds MZ twins P-O Cov (Xi,Xj) = ¼ Cov(MZ) + ½ Cov(P-O) + ¼ Cov(Unrel) = ¼(VAQTL+VDQTL) + ½ (½ VAQTL) + ¼ (0) = ½ VAQTL + ¼VDQTL

  47. Summary

  48. Biometrical model predicts contribution of a QTL to the mean, variance and covariances of a trait 1 QTL = VAQTL+ VDQTL Var (X) = VAQTL+ VDQTL Cov (MZ) = ½VAQTL+ ¼VDQTL Cov (DZ) Multiple QTL = Σ(VAQTL)+ Σ(VDQTL) = VA + VD Var (X) Cov (MZ) = Σ(VAQTL)+ Σ(VDQTL) = VA + VD Cov (DZ) = Σ(½VAQTL)+ Σ(¼VDQTL) = ½VA + ¼VD

  49. Biometrical model underlies the variance components estimation performed in Mx = VA + VD + VE Var (X) Cov (MZ) = VA + VD Cov (DZ) = ½VA + ¼VD

  50. 1/3 Biometrical model for single biallelic QTL 2A. Average allelic effect (α) The deviation of the allelic mean from the population mean Allele a Population Allele A a(p-q) + 2pqd ? ? Mean (X) a A αa αA AA Aa aa Allelic mean Average allelic effect (α) a d -a Ap qap+dqq(a+d(q-p)) a p qdp-aq-p(a+d(q-p))

More Related