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NAJRAN UNIVERSITY College of Applied Medical Sciences

NAJRAN UNIVERSITY College of Applied Medical Sciences. General Microbiology Course Lecture No. 6. By. Dr. Ahmed Morad Asaad Associate Professor of Microbiology. Bacterial Genetics. The nucleic acid is composed of: 1- Phosphoric acid

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NAJRAN UNIVERSITY College of Applied Medical Sciences

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  1. NAJRAN UNIVERSITY College of Applied Medical Sciences General Microbiology Course Lecture No. 6 By Dr. Ahmed MoradAsaad Associate Professor of Microbiology

  2. Bacterial Genetics

  3. The nucleic acid is composed of: • 1- Phosphoric acid • 2- Sugar (pentose) residue: Ribose (in RNA) or deoxyribose (in DNA). • 3- Nitrogenous bases: • *- Purine bases {adenine (A) and guanine (G)} • *- Pyrimidine bases {thymine (T), cytosine (C) and uracil (U)}.

  4. Bacterial chromosome Codon A in one chain – T in other chain Backbone of phosphate and deoxyribose groups C in one chain – G in other chain Hydrogen bonds between each pair of bases

  5. DNA replication RNA primer 5’ G A T C C T T T C G A A 3’ 3’ C T A G G A A A G C T T 5’ 5’ G A T C C T T T C G A A 3’ 3’ C T A G G A A A G C T T 5’ RNA primer

  6. Gene expression 5’ G A T C C T T T C G A A 3’ 3’ C T A G G A A A G C T T 5’ 5’ G A T C C T T T C G A A 3’ 3’ C T A G G A A A G C T T 5’ Transcription Messenger RNA (m RNA)

  7. Translation • The m RNA passes out of the nuclear body and is associated with the ribosome to form a ribosomal template on which amino acids will be arranged. • Amino acids are carried by specific RNA molecule called transfer RNA (tRNA) • Each tRNA carries an amino acid by one end and recognizes a specific codon on the mRNA by a complementary triplet (anticodon) present on its other end. • After the process of lining up of amino acids is completed, the amino acids are joined by peptide bonds to form a polypeptide chain. Assembly of polypeptide chains to form proteins and macromolecules then occurs.

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