1. Faculty of allied medical sciences

2. Lab SAFETY Dr. Eman El-Attar MLCC 203

3. ILOs Understand Safety program KnowLabhazards Know Safety Equipment To know spill response To know safety symbols

4. Safe laboratory procedures and training are needed for everyone!

5. Lab safety must be taught to : • all employees, including service groups • Undergraduate & graduate students • professors • visitors

6. REVIEW LABORATORY SAFETY WHEN: • new employees • new procedures • a change in procedures • new equipment

7. 10 activities leading to errors • Time pressure • Distracted environment • High workload • First time • First working day after days off

8. Half an hour after wake up or meals • Absence of supervision • Over confidence • Communication errors • Work stress

9. Types of Lab hazards • Biological • Chemical • Fire • Electrical • Radiological

10. LABORATORY PROCEDURES MUST BE SITE SPECIFIC ! based on your lab needs, conditions, and equipment

11. General consideration when design a lab • Location? • Rooms? • Ventilation? • Signs ? • Type of lab : Pathology, Chemistry, Biology, Radiation,….. ?

12. TYPES OF LABORATORIES • Pathology • Chemistry • Biology • Radiation

13. Laboratory Policies and Procedures Must be: written and available

14. General Rules: • no food or drink allowed in the laboratory • no mouth pipetting • individuals not allowed to work in the laboratory alone • wear required personal protective equipment • no smoking in laboratory • maintain good housekeeping habits

15. General Rules: • Open toed shoes and sandals, jewelery, loose clothes are not acceptable footwear • Hair and scarves should be tied back • Clothes : natural fibers as cotton • Wear required personal protective equipment

16. General Rules: • Report ALL accidents immediately, even if you think it is minor • Chemicals must be labeled for : contents, hazards, name of owner and date. • Before you leave your work area each day, check : • Overnight operations are properly set-up • Waterlines are adequately clamped • Heat sources & electricity are turned off • Hoods are closed • Chemicals and biological agents are stored properly

17. General Rules: • Remove your gloves before using instruments, telephone, and leaving the laboratory • Never suck pens or chew pencils • Always wash your hands before you leave and especially before eating • Never touch your face, mouth or eyes

18. GLASSWARE PROCEDURES • Storage • Proper use • Cleaning • Cleaning up broken glassware • Disposal of broken glassware

19. Glassware Handling Is this what your lab looks like?

20. Glassware handling cont • Or does it look like this?

21. Procedures for handling chemicals • proper labeling, including wastes • proper storage • storage cabinets • store compatible chemicals together • rooms properly vented & correct temperature

22. Maintain a current inventory • Purchasing procedures • Proper handling • use label or MSDS • never test by taste or odor • acids poured into water never vice versa • take precautions & use proper equipment when stirring or heating flammable liquids • follow “industry standards” for labeling all chemicals • Heavy bottles are put on the ground light ones put on shelves.

23. Chemicals are generally divided into: • Flammables. • Oxidizers. • Explosives. • Acids. • Bases. • Toxic. • Combustible.

24. Placing chemicals according to the following rules: • Flammable : below and away from heat • Oxidizers: away from Acids. • Explosives : away from heat • Acids: good ventilation & away from bases. • Bases : away fom acids & H2O • Toxic • Combustible : away from heat

25. Handling and Using Lab Equipment • Proper installation • Training on proper use • Manuals or written procedures available • Inspections • Maintenance • DOCUMENT

26. EQUIPMENT INCLUDES: • Meters • Refrigerators • Autoclaves • Scales • Hoods • Drying oven • Compressed gas cylinders • Bunsen burners

27. Lab Equipment Which hood ?

28. SAFETY EQUIPMENT • proper equipment must be available • training for the location so individuals know how and when to use equipment appropriately • training on the correct maintenance & storage

29. SAFETY EQUIPMENT • First aid & medical treatment • Emergency equipment • Showers, Eye wash • MSDS • PPE

30. 1- First Aid and Medical Treatment • First aid kit available and properly stocked (nothing expired) • Trained first aid attendant or • Medical facilities within 15 minutes • Emergency numbers posted

31. 2- EmergencyEquipment • Fire blankets • Fire extinguishers • Emergency notification system • Unrestricted means of communication • Any other emergency equipment needed for yourspecificlaboratoryneeds

32. 3- Emergency Showers & Eyewash Stations • Immediate washing of the skin and eye with generous amounts of water is the most effective first aid treatment for chemical burns (unless chemical reacts unfavorably to water-MSDS)

33. Emergency Showers and Eyewash Stations • must be available • showers must be tested for proper operation with results documented

34. Alternatives : • Portable showers or eyewashes • Attachments that connect to existing faucets • must supply at least 15 minutes of continuous water • must stay on until turned off

35. 4- Material Safety Data Sheets, (MSDS) • required for each chemical • must be accessable for employees/student • Must be reviewed MSDS FILE

36. MSDS Information Includes: • Nomenclature including chemical family and formula • Hazardous ingredients • Physical data • Fire and explosion hazard • Health hazard • Spill and leak procedures • Special protection information • Storage and handling precautions

40. 5- Personal Protective Equipment PPE Lab MUST provide PPE for all employees at no cost to the employee • Train them how to use PPE properly • Train them on the limitation(s) of PPE • train them on proper care, storage, and useful life, and disposal of PPE

41. Appropriate PPE: • aprons,lab coats • Gloves: latex,nitrile,neoprene • goggles, face shields, safety glasses • respirators, dust mask • noise protection

42. SPILLAGE • Clear up spillage promptly • You will already have determined how to do this as part of your risk assessment • Dispose of any hazardous material as toxic waste Messy workers are usually poor workers!!

43. ELECTRICAL SAFETY • Protection of employees and equipment • inspect panels and plugs • GFIs (specified by code) • surge protectors • inspection & reporting programs

44. FIRE SAFETY • Fire Burns – Stop, Drop, and be covered with a fire blanket or soaked with water. Do not remove clothing stuck to victim. Get medical attention immediately. • Make sure corridor fire doors and laboratory doors are kept shut at all times

45. DISPOSAL PROCEDURES • chemical wastes • organisms, diseases, animals • glassware • spills • sharps

46. DISPOSAL PROCEDURES CONT. • Trained persons to handle disposal • Meet all required rules and regulations • Proper collection containers • Waste collection contracts • Does not expose humans, animals, plants, etc upon disposal - may include decontamination, sterilization, incineration, autoclaving

47. EMERGENCY RESPONSE PLAN • Labs should develop an emergency response plan BEFORE an emergency • review with employees (students) make sure they understand the plan completely

48. Emergency Response Plan should include: • recognizing emergencies • lines of authority • methods of communication • safe sites and evacuation routes • site security and control

49. INSPECTION OF LABORATORIES • Develop inspection report appropriate for laboratory • cover all areas related to laboratory • personnel practices • operational practices • equipment • emergency protection equipment • materials inventory • miscellaneous

50. Lab Safety Symbols • Glassware • Heat • Eye and Face • Sharps • Electrical • Animal • Chemical • Fire